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DOI :
10.3791/56080-v
July 24th, 2017
Chapters
0:05
Title
0:40
Preparation of Competent Cells
2:40
Co-transformation of Cells by Heat Shock
4:39
Transferring Cells onto New Plates
5:25
Preparing Culture for Measuring β-galactosidase Activity
8:14
Measuring β-galactosidase Activity
9:07
Results: Y1.5H Assay Detects Heteromeric Protein-DNA Interactions
9:50
Conclusion
这里描述的修饰的酵母单杂交测定法是经典酵母单杂交(Y1H)测定法的扩展,以研究和验证异源蛋白复合物 - DNA异构体系中任何功能基因组学研究的相互作用。
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