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DOI :
10.3791/56096-v
November 2nd, 2017
Chapters
0:05
Title
0:54
Cell Culture and Cell Cycle Synchronization
3:18
Immunoprecipitation of Endogenerous Rb Protein
6:12
Analysis of 6XHis-tagged Exogenous Rb SUMOylation in Human Cells
8:20
Results: Detection of Both Endogenous and Exogenous Rb SUMOylation
9:08
Conclusion
小さなユビキチン関連の修飾子 (相撲) ファミリー蛋白質は様々 な細胞プロセスを調整するターゲット蛋白質のリジン残基に抱合します。本稿では、rb タンパク質 sumo 化ひと細胞の内因性と外因性の条件下での検出のためのプロトコルについて説明します。
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