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DOI :
10.3791/56100-v
October 3rd, 2017
Chapters
0:05
Title
1:24
Introduction to the Accompanying Publication
2:47
Nematode Fixation
4:19
Nematode Antibody Staining
6:33
Applying RNAi to Nematodes Using a Feeding Protocol
7:57
Analyzing Phenotypes Using Fluorescence Dissecting Microscopy
8:41
Analyzing Phenotypes Using Confocal Microscopy
10:14
Results: Immunohistochemistry, RNAi and Microscopic Analysis of Intestinal Apical membrane and Lumen Morphogenesis
11:45
Conclusion
透明的C. 线虫小肠可作为 "体内组织室", 用于研究多细胞 tubulogenesis 中的单细胞和亚 apicobasal 膜和腔生物。本协议描述了如何结合标准标记, 功能的遗传/rna 干扰和微观的方法来解剖这些过程的分子水平。
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