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C. 线虫排泄管作为细胞内腔形态发生和体内极化膜生物在单个细胞中的模型: GFP-融合、rna 干扰界面和成像的标记

DOI :

10.3791/56101-v

October 3rd, 2017

October 3rd, 2017

9,409 Views

1Mucosal Immunology and Biology Research Center, Developmental Biology and Genetics Core, Massachusetts General Hospital for Children, Harvard Medical School, 2College of Life Sciences, Jilin University, 3Faculty of Health Sciences, University of Macau

C. 线虫排泄管是一种独特的单细胞模型, 用于可视化的体内分析从头生物极化膜。该协议描述了标准的遗传/rna 干扰和成像方法相结合, 适用于定向单细胞 tubulogenesis、顶端膜和腔生物的分子的鉴定和表征。

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