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DOI :
10.3791/56101-v
October 3rd, 2017
Chapters
0:05
Title
1:02
Introduction to the Accompanying Publication
2:28
RNAi Interaction Screen to Modify a Canal lumen Phenotype for Identification of Genes Involved in Intracellular Lumen Extension
3:29
Scoring Excretory Canal Phenotypes using Fluorescence Dissecting Microscopy
5:20
Imaging the C. elegans Excretory Canal using Confocal Microscopy
8:16
Results: GFP Labeling, RNAi and Microscopic Analysis of Excretory Canal Lumen Morphogenesis Phenotypes
9:51
Conclusion
線虫 c. エレガンスの排泄管はde novo偏光膜生合成の視覚的生体内解析のためのユニークな単一細胞モデルです。このプロトコルでは、標準的な遺伝学的/RNAi とアプローチ、同定と解析分子単細胞 tubulogenesis と頂端膜と内腔を演出するための適応をイメージングの組み合わせについて説明します。
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