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DOI :
10.3791/56106-v
•
8:31 min
August 2nd, 2017
Chapters
0:05
Title
0:55
Preparation of Skin Samples
2:07
Cryosectioning
3:17
Immunofluorescent Labeling
5:05
Mounting for Microscopic Visualization
6:36
Results: Horizontal Whole Mount Compared to a Classical Cryosection
7:55
Conclusion
この研究は、共焦点イメージングモダリティの完全な利用を可能にする、厚い3次元組織断面分析のための新規な処理およびイメージングプロトコルを提示する。このプロトコールは抗原性を保持し、皮膚組織学および潜在的に他の組織型を分析するための堅牢なシステムを表す。
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