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DOI :
10.3791/56113-v
•
12:28 min
November 10th, 2017
Chapters
0:05
Title
1:12
Gelatin Infiltration, Mounting, and Cryosectioning
3:47
Immunolabeling
5:26
Super-resolution Microscopy
7:25
Platinum Shadowing and Scanning Electron Microscopy
8:48
Alignment of Light and Electron Microscopy Images
10:23
Results: Tom20 Protein Expression in the Zebrafish Retina
11:29
Conclusion
该协议描述了在斑马鱼视网膜上获得亚细胞蛋白定位结果的必要步骤, 方法是将超分辨光显微镜和扫描电子显微镜图像关联起来。
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