Experiment Preparation in a BSL-2 Environment Prior to Working with the Virus Samples (Biocontainment Procedures were Filmed in BSL-4 Training Lab)
1:45
The Capsule Method for Negative Staining in Biocontainment Using Aqueous Glutaraldehyde and 1% Osmium Tetroxide Vapor Inactivation
4:24
The Capsule Method for Inactivation in Biocontainment 2% Glutaraldehyde Followed by Negative Staining in BSL-2 Laboratory
5:50
Results: Analyzing the Capsule Method of Negative Staining Viral Samples
7:14
Conclusion
Transcript
The overall goal of this method is to provide a simple and effective TEM negative staining procedure for viral samples within biocontainment. This method can help answer key questions in virology about the morphology and structures of viruses bein
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This protocol provides instruction for negative staining virus samples which can easily be used in BSL-2, -3, or -4 laboratories. It includes the use of an innovative processing capsule, which protects the transmission electron microscopy grid and provides the user easier handling in the more turbulent environments within biocontainment.