Utilization of Capsules for Negative Staining of Viral Samples within BiocontainmentCandace D. Blancett 1, Mitchell K. Monninger 1, Chrystal A. Nguessan 1, Kathleen A. Kuehl 1, Cynthia A. Rossi 2, Scott P. Olschner 2, Priscilla L. Williams 2, Steven L. Goodman 3, Mei G. Sun 1
1Pathology Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 2Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 3Microscopy Innovations LLC
This protocol provides instruction for negative staining virus samples which can easily be used in BSL-2, -3, or -4 laboratories. It includes the use of an innovative processing capsule, which protects the transmission electron microscopy grid and provides the user easier handling in the more turbulent environments within biocontainment.
