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DOI :
10.3791/56130-v
September 22nd, 2017
Chapters
0:05
Title
0:57
Fresh Tissue Sectioning
2:25
Primary, Secondary, and Tertiary Antibody (Ab) Incubation
4:37
Sample Mounting and Confocal Microscopic Imaging
5:47
Results: Representative MHC-tetramer Staining
7:25
Conclusion
在这里, 我们描述一个方法, 结合原位MHC 聚丙烯染色与免疫组化, 以确定的定位, 表型和数量的抗原特异性 T 细胞在组织。本协议用于确定抗原特异性 CD8 T 细胞相对于其他细胞类型和组织结构的空间和表型特征。
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