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DOI :
10.3791/56130-v
September 22nd, 2017
Chapters
0:05
Title
0:57
Fresh Tissue Sectioning
2:25
Primary, Secondary, and Tertiary Antibody (Ab) Incubation
4:37
Sample Mounting and Confocal Microscopic Imaging
5:47
Results: Representative MHC-tetramer Staining
7:25
Conclusion
ここでは、免疫組織化学的局在、表現型、組織における抗原特異的 T 細胞の量を決定するとその場でMHC テトラマー染色を組み合わせた手法について述べる。このプロトコルは、他のセルの種類および組織の構造を基準にして抗原特異的 CD8 T 細胞の表現型特性を決定する使用です。
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