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DOI :
10.3791/56144-v
August 17th, 2017
Chapters
0:05
Title
0:37
Phage Amplification
2:15
Purification of Phage
5:39
Double-stranded Genome DNA Extraction
8:19
Cell-free Phage Reaction and Phage Titer Experiment
9:35
Results: Synthesis and Quantification of Phage T7 in Varying Molecular-crowding Environments
10:46
Conclusion
無細胞転写・翻訳プラットフォームの新世代は、生化学システムの in vitro遺伝子回路の実行を構築するために設計されています。この記事で私たちはすべて大腸菌無細胞 TXTL システムを使用して彼らのゲノムからのバクテリオファージ MS2、ΦΧ174、T7 などの合成方法について説明します。
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