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DOI :
10.3791/56187-v
October 27th, 2017
Chapters
0:05
Title
1:04
Generating Embryos Through Natural Mating and Staging Embryos
2:22
Whole Embryo Dissociation, RNA Extraction and Purify High-quality RNA
4:55
Sorting Differentially Expressed Genes
7:00
Determine Enriched Pathways
7:57
Generation of Pathway Networks and Determination of Enriched GO Terms
9:12
Results: RNAseq-based Zebrafish Genome Expression Data for Alstrom and Bardet Biedl Syndromes
10:47
Conclusion
이 프로토콜에서 zebrafish 태아, 애벌레, 전체 transcriptome 분석에 대 한 접근을 제공 하거나 셀을 정렬 합니다. 우리는 RNA의 격리, RNASeq 데이터의 통로 분석 및 유전자 표정 변화의 qRT-PCR 기반 유효성 검사를 포함합니다.
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