Dissektion af menneskelige nethinde og RPE-choroidea proteom analyse
November 12th, 2017
•Thiago Cabral1,2,7,8*, Marcus A. Toral3,4*, Gabriel Velez3,4, James E. DiCarlo1,2, Anuradha M. Gore3, MaryAnn Mahajan3, Stephen H. Tsang1,2, Alexander G. Bassuk5,6, Vinit B. Mahajan3,9
1Barbara & Donald Jonas Stem Cell Laboratory, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons, Columbia University, 2Edward S. Harkness Eye Institute, New York-Presbyterian Hospital, 3Omics Laboratory, Byers Eye Institute, Department of Ophthalmology, Stanford University, 4Medical Scientist Training Program, University of Iowa, 5Department of Pediatrics, University of Iowa, 6Department of Neurology, University of Iowa, 7Department of Ophthalmology, Federal University of Sao Paulo (UNIFESP), 8Department of Ophthalmology, Federal University of EspÍrito Santo (UFES), 9Palo Alto Veterans Administration, Palo Alto, CA
Den menneskelige nethinde består af funktionelt og molekylært forskellige regioner, herunder fovea, gule plet og perifere retina. Her, beskriver vi en metode ved hjælp af punch biopsier og manuel fjernelse af væv lag fra en menneskelige øje at dissekere og indsamle disse særskilte retinal regioner for downstream proteom analyse.
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