Dissezione della Retina umana e RPE-coroide per analisi proteomica
November 12th, 2017
•Thiago Cabral1,2,7,8*, Marcus A. Toral3,4*, Gabriel Velez3,4, James E. DiCarlo1,2, Anuradha M. Gore3, MaryAnn Mahajan3, Stephen H. Tsang1,2, Alexander G. Bassuk5,6, Vinit B. Mahajan3,9
1Barbara & Donald Jonas Stem Cell Laboratory, and Bernard & Shirlee Brown Glaucoma Laboratory, Department of Pathology & Cell Biology, Institute of Human Nutrition, College of Physicians and Surgeons, Columbia University, 2Edward S. Harkness Eye Institute, New York-Presbyterian Hospital, 3Omics Laboratory, Byers Eye Institute, Department of Ophthalmology, Stanford University, 4Medical Scientist Training Program, University of Iowa, 5Department of Pediatrics, University of Iowa, 6Department of Neurology, University of Iowa, 7Department of Ophthalmology, Federal University of Sao Paulo (UNIFESP), 8Department of Ophthalmology, Federal University of EspÍrito Santo (UFES), 9Palo Alto Veterans Administration, Palo Alto, CA
La retina umana è composta in modo funzionale e molecolare distinte regioni, tra cui la fovea, la macula e la retina periferica. Qui, descriviamo un metodo utilizzando biopsie punch e rimozione manuale di strati di tessuto da un occhio umano per sezionare e raccogliere queste regioni distinte della retina per analisi proteomica a valle.
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