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DOI :
10.3791/56214-v
August 9th, 2017
Chapters
0:05
Title
0:48
Embryo Collection for Time-lapse Imaging
1:51
Embryo Preparation
2:52
Preparation of 2% Low-melt Agarose and Mounting the Embryo
4:34
Adjusting Software Settings on the Multi-photon Microscope and Time-lapse Acquisition
7:26
Post-acquisition Processing
8:10
Results: Multi-photon Time-lapse Imaging of Migrating Neural Crest Cells in Zebrafish Embryos
9:29
Conclusion
激光扫描与波长多光子荧光显微镜的先进光学技术组合的实施是为了捕获高分辨率的、 三维的、 实时成像中甘油三酯 (sox10:EGFP) 和甘油三酯 (foxd3:GFP) 斑马鱼胚胎神经嵴迁移。
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