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DOI :
10.3791/56214-v
August 9th, 2017
Chapters
0:05
Title
0:48
Embryo Collection for Time-lapse Imaging
1:51
Embryo Preparation
2:52
Preparation of 2% Low-melt Agarose and Mounting the Embryo
4:34
Adjusting Software Settings on the Multi-photon Microscope and Time-lapse Acquisition
7:26
Post-acquisition Processing
8:10
Results: Multi-photon Time-lapse Imaging of Migrating Neural Crest Cells in Zebrafish Embryos
9:29
Conclusion
長い波長多光子蛍光励起顕微鏡レーザーの高度な光学技術の組み合わせは、Tg (sox10:EGFP) および Tg (foxd3:GFP) ゼブラフィッシュ胚の神経堤移行の高解像度、リアルタイム三次元画像をキャプチャする実装されました。
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