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DOI :
10.3791/56248-v
December 7th, 2017
Chapters
0:05
Title
0:38
siRNA Transient HIV-Tat-interactive Protein 60 kDa (TIP60) Depletion
2:08
Wound-healing Assay Cell Seeding and TIP60 Knockdown Efficiency Evaluation
4:10
Wound Creation, Imaging, and Single Cell Movement Tracking
6:32
Results: Representative Videos and Quantifications of siTIP60 and siControl Cell Migration
7:49
Conclusion
在这里, 我们提出了实时监测细胞迁移在伤口愈合试验中使用 TIP60-depleted MCF10A 乳腺上皮细胞。在我们的协议中实现了活细胞成像技术, 使我们能够实时和跨时间地分析和可视化单细胞运动。
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