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DOI :
10.3791/56260-v
November 8th, 2017
Chapters
0:05
Title
0:52
Generation of Human iPSCs from Primary Human Pancreatic Cells
3:41
Genome Editing of iPSC Using CRISPER-Cas9
5:45
Single-cell Isolation of Targeted hiPSCs
7:15
Results: Editing of Feeder-free iPSCs with CRISPER-Cas9 System
8:29
Conclusion
本协议详细描述了在无饲养条件下从人类胰腺细胞中生成无足迹诱导多能干细胞 (干细胞), 其次是使用 CRISPR/Cas9 均一进行编辑和修饰单细胞克隆。
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