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DOI :
10.3791/56281-v
•
14:21 min
October 19th, 2017
Chapters
0:05
Title
1:09
PCR Amplification of cDNA Templates from Plasmids
3:43
In vitro Transcription to Generate Antisense Probes
5:25
Drosophila Tissue Collection and Fixation
7:51
In Situ Hybridization
9:05
Probe Detection
11:05
Antibody Detection using Tyramide
12:01
Results: Robust Detection of RNA in Whole Drosophila Embryos and Dissected Tissues
13:02
Conclusion
교 잡 프로토콜 설명된 RNA 제자리에 전체 초파리 배아 또는 해 부 조직에서 RNA의 검출을 허용 한다. 96-잘 결정 접시와 tyramide 신호 증폭에 사용 하 여, 성적 감지할 수 있습니다 높은 해상도, 감도, 및 처리량, 그리고 상대적으로 저렴 한 비용.
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