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DOI :
10.3791/56302-v
October 4th, 2017
Chapters
0:05
Title
1:09
Polymerase Chain Reaction (PCR)-mediated Site-directed Mutagenesis
3:44
Uniform 15N-labeled Protein Expression in BL21 ΔE3 E. coli
5:24
Purification of Recombinant Protein from E. coli
7:50
Chemical Attachment of Glucose-5-MTS to Protein by Dialysis
8:48
Results: Site-selective Cys Mutation Followed by In Vitro Glycosylation in STIM1 EF-SAM Protein
10:11
Conclusion
糖タンパク質の生化学的および構造解析では、均質な試料の比較的大量を必要とします。反応システイン チオール化合物をターゲットに細菌から精製組換えタンパク質の部位特異的糖鎖の効率的な化学的方法を紹介します。
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