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DOI :
10.3791/56313-v
November 13th, 2017
Chapters
0:05
Title
0:54
Purification of Naïve Human CD4+ Cells, Polarization to Th1 and TH2 Cells, and Nuclei Extraction
3:18
Transposition Reaction and Library Amplification
4:33
Size Selection of ATAC-seq Libraries
6:48
Results: Quality Control and Next Generation Sequencing Reads
8:07
Conclusion
转-可获得的染色质结合高通量测序 (ATAC-seq) 是一个基因组范围的方法, 以揭示可获得的染色质。这是一个 step-by 步骤的 ATAC-seq 协议, 从分子到最终的计算分析, 优化为人类淋巴细胞 (Th1/Th2)。这一协议可以被研究人员采用, 而无需事先经验的下一代测序方法。
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