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DOI :
10.3791/56317-v
July 17th, 2018
Chapters
0:04
Title
0:33
Cell Transfection
2:08
Rh123 Labeling
4:21
Detection of the Mitochondrial Membrane Potential (MMP)
6:29
Confocal Laser Scanning Fluorescence Microscope
8:34
Statistical Analysis
9:48
Results: Detection of Mitochondrial Membrane Potential via Normal Fluorescent Microscopy and Confocal Laser Scanning Microscopy
11:53
Conclusion
在这里, 我们提出了一个详细的协议, 应用罗丹明123识别线粒体膜电位 (基质) 和研究 CLIC4 击倒诱导 HN4 细胞凋亡的体外。在普通荧光显微镜和共聚焦激光扫描荧光显微镜下, 记录了基质金属蛋白酶的实时变化。
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