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DOI :
10.3791/56333-v
November 23rd, 2017
Chapters
0:05
Title
1:09
Generation of Lentivirus Particles
2:34
Generation of Stably Transfected Lines
4:55
Isolation of Monocytes from Human Blood
8:00
Boyden Chamber Migration Assay
9:25
Results: PAI-1 Promotes Macrophage Migration
10:24
Conclusion
このプロトコルは、単球/マクロファージにおける腫瘍微小環境の募集に腫瘍細胞由来タンパク質の役割を研究するため特に癌細胞とその後続の使用で機能するテトにシステムを設定するためのスキームとして機能します。
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