Name: liquid-cell transmission electron microscopy for tracking self-assembly of nanoparticles
Uploaded: 2017-10-16T13:00:00
Description: Watch this Scientific Journal Video about Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles at JoVE.com

Ringraziamo la Prof. ssa A. Paul Alivisatos presso la University of California, Berkeley e Prof. ssa Taeghwan Hyeon alla Seoul National University per la discussione utile. Questo lavoro è stato supportato da IBS-R006-D1. W.C.L. riconosce con gratitudine il sostegno del fondo di ricerca di Hanyang University (HY-2015-N).

1. sintesi di nanoparticelle <ol><li>sintesi di nanoparticelle di platino <ol><li>combinare 17.75 mg di hexachloroplatinate(IV) di ammonio ((NH 4), 2 Cl di Pt (IV) 6), 3,72 mg di tetrachloroplatinate(II) di ammonio ((NH 4), 2 Cl di Pt (II) 4), 115,5 mg di bromuro di tetrametilammonio, 109 mg di poly(vinylpyrrolidone) (MW: 29.000) e 10 mL di glicole etilenico con un ancoretta a 100 mL con fondo tondo 3-collo dotato di un setto di gomma.<...

<ol>
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Nanoparticelle di platino con una dimensione di 7 nm sono stati sintetizzati attraverso la riduzione di ammonio esacloroplatinato (IV) ed ammonio tetrachloroplatinate (II) utilizzando poli (vinilpirrolidone) (PVP) come un ligando e il glicole etilenico come un solvente e un agente riducente27 . Una reazione di ligando-scambio con oleilammina è stata effettuata per disperdere le particelle in un solvente idrofobo. Le nanoparticelle di seleniuro di piombo sono state sintetizzate attraverso la decom...

L'auto-assemblaggio di nanoparticelle colloidali è di interesse perché offre l'opportunità di accedere a proprietà fisiche collettive di singole nanoparticelle11. Uno dei metodi più efficaci di auto-assemblaggio usato in applicazioni pratiche su scala periferica è auto-organizzazione delle nanoparticelle su un substrato mediante evaporazione di un solvente volatile6,7,8, 9 , 10 , 11. questo metodo di evaporazione del solvente è un processo di non-equilibrio, che è in gran parte influenzato da fattori cinetici come tasso di evaporazione e cambiamenti nelle interazioni di nanoparticelle-substrato. Tuttavia, poiché è difficile da stimare e controllare i fattori cinetici, la comprensione meccanicistica di nanoparticella autoassemblaggio di evaporazione del solvente non è completamente matura. Sebbene in situ x-ray scattering studi abbiano fornito informazioni una media di ensemble del nonequilibrium nanoparticella autoassemblaggio processo12,13,14, questa tecnica non può determinare il moto delle singole nanoparticelle, e la loro associazione con la traiettoria complessiva non può essere facilmente accessibili.
Liquido-cella TEM è uno strumento emergente per tracciare la traiettoria di singole nanoparticelle, permettendoci di capire la disomogeneità dei moti delle nanoparticelle e il loro contributo a ensemble comportamenti15,16, 17,18,19,20,21,22,23,24,25, 26. In precedenza abbiamo usato liquido-cella TEM per tracciare il movimento di singole nanoparticelle durante l'evaporazione del solvente, mostrando che il movimento del solvente limite è un'importante forza trainante per l'induzione della nanoparticella auto-assemblaggio su un substrato18 , 19. nel presente documento, vi presentiamo gli esperimenti dove possiamo osservare il processo di auto-assemblaggio utilizzando liquido-cella TEM di nanoparticelle. In primo luogo, forniamo protocolli per la sintesi di platino e piombo seleniuro nanoparticelle, prima di introdurre le procedure di fabbricazione di liquido-cellule per TEM e come caricare le nanoparticelle in liquido-cella. Come risultati rappresentativi, mostriamo immagini snapshot da film TEM di nanoparticella autoassemblaggio guidato da essiccamento solvente. Di monitoraggio delle singole particelle in questi film, possiamo capire i meccanismi dettagliati di solvente-asciugatura-mediata self-assembly a livello di singola nanoparticella. Durante l'auto-assemblaggio, le nanoparticelle di platino sulla finestra di nitruro di silicio seguono principalmente il movimento del fronte del solvente d'evaporazione a causa delle forti forze capillari agendo sul sottile strato solvente. Fenomeni simili sono stati osservati anche per altri substrati (silicio), che indica che la forza capillare del fronte del solvente è un fattore importante nella migrazione delle particelle nei pressi di un substrato e nanoparticelle (seleniuro di piombo).

<table>
	<tbody>
		<tr>
			<td>ammonium hexachloroplatinate (IV)</td>
			<td>Sigma-Aldrich</td>
			<td>204021</td>
			<td></td>
		</tr>
		<tr>
			<td>ammonium tetrachloroplatinate (II)</td>
			<td>Sigma-Aldrich</td>
			<td>206105</td>
			<td></td>
		</tr>
		<tr>
			<td>tetramethylammonium bromide, 98%</td>
			<td>Sigma-Aldrich</td>
			<td>195758</td>
			<td></td>
		</tr>
		<tr>
			<td>poly(vinylpyrrolidone) powder</td>
			<td>Sigma-Aldrich</td>
			<td>234257</td>
			<td>Mw ~29,000</td>
		</tr>
		<tr>
			<td>ethylene glycol, anhydrous, 99.8%</td>
			<td>Sigma-Aldrich</td>
			<td>324558</td>
			<td></td>
		</tr>
		<tr>
			<td>n-hexane, anhydrous, 95%</td>
			<td>Samchun Chem.</td>
			<td>H0114</td>
			<td></td>
		</tr>
		<tr>
			<td>ethanol, anhydrous, 99.5%</td>
			<td>Sigma-Aldrich</td>
			<td>459836</td>
			<td></td>
		</tr>
		<tr>
			<td>oleylamine, 70%</td>
			<td>Sigma-Aldrich</td>
			<td>O7805</td>
			<td>Technical grade</td>
		</tr>
		<tr>
			<td>lead(II) acetate trihydrate, 99.99%</td>
			<td>Sigma-Aldrich</td>
			<td>467863</td>
			<td></td>
		</tr>
		<tr>
			<td>oleic acid, 90%</td>
			<td>Sigma-Aldrich</td>
			<td>364525</td>
			<td>Technical grade</td>
		</tr>
		<tr>
			<td>diphenyl ether, 99%</td>
			<td>Sigma-Aldrich</td>
			<td>P24101</td>
			<td>ReagentPlus</td>
		</tr>
		<tr>
			<td>selenium powder, 99.99%</td>
			<td>Sigma-Aldrich</td>
			<td>229865</td>
			<td></td>
		</tr>
		<tr>
			<td>tri-n-octylphosphine, 97%</td>
			<td>Strem</td>
			<td>15-6655</td>
			<td>Air sensistive</td>
		</tr>
		<tr>
			<td>Toluene, anhydrous, 99.9%</td>
			<td>Samchun Chem.</td>
			<td>T2419</td>
			<td></td>
		</tr>
		<tr>
			<td>acetone 99.8%</td>
			<td>Daejung Chem.</td>
			<td>1009-2304</td>
			<td></td>
		</tr>
		<tr>
			<td>potassium hydroxide, 95%</td>
			<td>Samchun Chem.</td>
			<td>P0925</td>
			<td></td>
		</tr>
		<tr>
			<td>p-type silicon-on-insulator wafers</td>
			<td>Soitec</td>
			<td>Power-SOI</td>
			<td>for liquid cells with silicon windows</td>
		</tr>
		<tr>
			<td>tetramethylammonium hydroxide, 25% in H2O</td>
			<td>J.T.Baker</td>
			<td>02-002-109</td>
			<td></td>
		</tr>
		<tr>
			<td>AZ 5214 E</td>
			<td>AZ Electronic Materials</td>
			<td>AZ 5214 E</td>
			<td>Positive photorest</td>
		</tr>
		<tr>
			<td>AZ-327</td>
			<td>AZ Electronic Materials</td>
			<td>AZ-327</td>
			<td>AZ 5214 develper</td>
		</tr>
		<tr>
			<td>indium pellets 99.98-99.99%</td>
			<td>Kurt J. Lesker Company</td>
			<td>EVMIN40EXEB</td>
			<td>thermal evaporator target</td>
		</tr>
		<tr>
			<td>1,2-dichlorobenzene, &#62;99%</td>
			<td>TCI</td>
			<td>D1116</td>
			<td></td>
		</tr>
		<tr>
			<td>pentadecane, &#62;99%</td>
			<td>Sigma-Aldrich</td>
			<td>P3406</td>
			<td></td>
		</tr>
		<tr>
			<td>buffered oxide etch 7:1</td>
			<td>microchemicals</td>
			<td>BOE 7-1 VLSI</td>
			<td></td>
		</tr>
		<tr>
			<td>phosphoric acid, 85%</td>
			<td>Samchun Chem.</td>
			<td>P0449</td>
			<td></td>
		</tr>
	</tbody>
</table>

liquid-cell transmission electron microscopy for tracking self-assembly of nanoparticles

Una dispersione di nanoparticelle di essiccazione è un modo versatile per creare strutture auto-assemblate di nanoparticelle, ma il meccanismo di questo processo completamente non è capito. Noi abbiamo tracciato le traiettorie delle singole nanoparticelle mediante microscopia elettronica a trasmissione di liquido-cella (TEM) per studiare il meccanismo del processo assembleare. Qui, presentiamo i protocolli usati per liquidi-cella TEM del meccanismo di auto-assemblaggio. In primo luogo, vi presentiamo i protocolli dettagliati di sintetici utilizzati per produrre dimensione platino e nanoparticelle di seleniuro di piombo. Successivamente, vi presentiamo i processi di microfabbricazione utilizzati per produrre le cellule di liquide con nitruro di silicio o silicio windows e poi descrivere il carico e le procedure della tecnica liquido-cella TEM di imaging. Varie note sono incluse per fornire consigli utili per l'intero processo, incluse le modalità di gestione delle finestre della cella fragile. I singoli movimenti di nanoparticelle rilevate da liquido-cella TEM ha rivelato che cambiamenti nei confini solventi causati da evaporazione influenzato il processo auto-assemblaggio delle nanoparticelle. I confini di solventi ha spinto le nanoparticelle a principalmente aggregati amorfi di forma, seguiti da appiattimento degli aggregati per produrre una struttura auto-assemblata di 2-dimensionale (2D). Questi comportamenti si osservano anche per tipi diversi di nanoparticelle e diverse composizioni di liquido-cella.

Il liquido-cella è composta da un chip superiore e un chip di fondo, che sono dotati di finestre di nitruro di silicio che sono trasparenti per un fascio di elettroni con uno spessore di 25 nm. Il chip superiore ha un serbatoio per la conservazione della soluzione del campione e solvente evaporato. I chip sono realizzati in via convenzionale microfabbricazione elaborazione25. Le maschere utilizzate per i chip superiore e inferiore sono mostrate in <strong class="x...

Watch this Scientific Journal Video about Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles at JoVE.com

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles

Microscopia elettronica in trasmissione di liquidi-cellula per l'inseguimento auto-assemblaggio delle nanoparticelle

Qui introduciamo protocolli sperimentali per l'osservazione in tempo reale di un processo auto-assemblaggio mediante microscopia elettronica a trasmissione di liquido-cella.

Drying a nanoparticle dispersion is a versatile way to create self-assembled structures of nanoparticles, but the mechanism of this process is not fully ...

Research

JoVE Journal

Chemistry

Origami Inspired Self-assembly of Patterned and Reconfigurable Particles

Origami Ispirato auto-assemblaggio di particelle Fantasia e riconfigurabile

There are numerous techniques such as photolithography, electron-beam lithography and soft-lithography that can be used to precisely pattern two ...

Ricerca

Chimica

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Formazione di strutture biomolecolari ordinate dalla Self-assemblaggio di piccoli peptidi

In nature, complex functional structures are formed by the self-assembly of biomolecules under mild conditions. Understanding the forces that control ...

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Auto-assemblaggio di forme complesse bidimensionali da Campagna DNA a singolo filamento

Current methods in DNA nano-architecture have successfully engineered a variety of 2D and 3D structures using principles of self-assembly. In this ...

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography

Come ottenere mappe 3D chimiche di energia filtrata tomografia di microscopia elettronica di trasmissione

Energy filtered transmission electron microscopy tomography (EFTEM tomography) can provide three-dimensional (3D) chemical maps of materials at a ...

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Analisi di minerali prodotto da hFOB 1.19 e Saos-2 cellule mediante microscopia elettronica a trasmissione con microanalisi a raggi x dispersiva di energia

This video presents the use of transmission electron microscopy with energy dispersive X-ray microanalysis (TEM-EDX) to compare the state of minerals in ...

Using Graphene Liquid Cell Transmission Electron Microscopy to Study in Situ Nanocrystal Etching

Using Graphene Liquid Cell Transmission Electron Microscopy to Study in Situ Nanocrystal Etching

Usando la microscopia elettronica di trasmissione di Graphene liquido cellulare per studiare in Situ nanocristallo acquaforte

Graphene liquid cell electron microscopy provides the ability to observe nanoscale chemical transformations and dynamics as the reactions are occurring in ...

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Eseguire la spettroscopia su nanoparticelle plasmoniche con microscopia a contrasto di tipo Nomarski basata su trasmissione

Differential interference contrast (DIC) microscopy is a powerful imaging tool that is most commonly employed for imaging microscale objects using ...

Preparation of Graphene-Supported Microwell Liquid Cells for In Situ Transmission Electron Microscopy

Preparation of Graphene-Supported Microwell Liquid Cells for In Situ Transmission Electron Microscopy

Preparazione di cellule liquide microwell supportate al grafene per la microscopia elettronica di trasmissione in situ

The fabrication and preparation of graphene-supported microwell liquid cells (GSMLCs) for in situ electron microscopy is presented in a stepwise protocol. ...

Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers

Sintesi di nanoparticelle cilindriche monodisperse tramite l'autoassemblaggio di copoliomeri a blocchi biodegradabili

The production of monodisperse cylindrical micelles is a significant challenge in polymer chemistry. Most cylindrical constructs formed from diblock ...

Self-Assembly of Hybrid Lipid Membranes Doped with Hydrophobic Organic Molecules at the Water/Air Interface

Auto-assemblaggio di membrane lipidiche ibride drogate con molecole organiche idrofobiche presso l'interfaccia acqua/aria

Because of their unique properties, including an ultrathin thickness (3-4 nm), ultrahigh resistivity, fluidity and self-assembly ability, lipid bilayers ...