Name: liquid-cell transmission electron microscopy for tracking self-assembly of nanoparticles
Uploaded: 2017-10-16T13:00:00
Description: Watch this Scientific Journal Video about Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles at JoVE.com

Vi takker Prof A. Paul Alivisatos ved University of California, Berkeley og Prof Taeghwan Hyeon ved Seoul National University for nyttig diskusjonen. Dette arbeidet ble støttet av IBS-R006-D1. W.C.L. anerkjenner takknemlig støtte fra research fund Hanyang University (HY-2015-N).

1. syntese av nanopartikler <ol><li>syntese av platina nanopartikler <ol><li>kombinerer 17.75 mg ammonium hexachloroplatinate(IV) ((NH 4) 2 Pt (IV) Cl 6), 3,72 mg ammonium tetrachloroplatinate(II) ((NH 4) 2 Pt (II) Cl 4), 115.5 mg tetramethylammonium bromide, 109 mg poly(vinylpyrrolidone) (MW: 29,000), og 10 mL etylenglykol med rør bar i en 100 mL 3-hals runde bunnen kolbe utstyrt med en gummi septum.</li>
		<li>Utstyr flasken med en r...

<ol>
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H., Choi, S., Takeuchi, S., Park, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Liquid+Cell+Electron+Microscopy+of+Nanoparticle+Self-Assembly+Driven+by+Solvent+Drying.">Liquid Cell Electron Microscopy of Nanoparticle Self-Assembly Driven by Solvent Drying.</a> J. Phys. Chem. Lett. 8, 647-654 (2017).</li><li>Park, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=3D+Structure+of+Individual+Nanocrystals+in+Solution+by+Electron+Microscopy.">3D Structure of Individual Nanocrystals in Solution by Electron Microscopy.</a> Science. 349, 290-295 (2015).</li><li>Chee, S. W., Baraissov, Z., Loh, N. D., Matsudaira, P. 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Platina nanopartikler størrelse 7 nm ble syntetisert via reduksjon av ammonium hexachloroplatinate (IV) og ammonium tetrachloroplatinate (II) med poly (vinylpyrrolidone) (PVP) som en ligand og etylenglykol som et løsemiddel og et reduksjonsmiddel27 . En ligand-utveksling reaksjon med oleylamine ble utført for å spre partikler i et hydrofobe løsemiddel. Bly selen nanopartikler ble syntetisert via Termisk nedbrytning av bly-oleate komplekser med topp-Se som en selen kilde28</s...

Den selv-montering av kolloidalt nanopartikler er rundt fordi det gir en mulighet til kollektiv fysiske egenskaper av personlige nanopartikler11. En av de mest effektive metodene for selv-montering brukes i praktisk enhet skala programmer er selv-organisering av nanopartikler på et substrat gjennom fordamping av flyktige løsemiddel6,7,8, 9 , 10 , 11. dette løsemiddel fordampning metoden er en nonequilibrium prosess, som er i stor grad påvirket av kinetic faktorer som Fordampningshastighet og endringer i hydrogenion-underlaget interaksjon. Men siden det er vanskelig å beregne og kontrollere kinetic faktorene, er mekanistisk forståelsen av hydrogenion selvstendig montering av løsemiddel fordampning ikke fullt ut modne. Selv om i situ X-ray spredning studier har gitt ensemble-gjennomsnitt informasjon av nonequilibrium hydrogenion selvtillit forsamlingen behandle12,13,14, denne teknikken kan ikke bestemme bevegelse av individuelle nanopartikler, og tilknytningen samlede banen ikke kan nås enkelt.
Væske-celle TEM er en nye verktøy for sporing banen for individuelle nanopartikler, slik at vi kan forstå inhomogeneity av hydrogenion bevegelser og deres bidrag til ensemble atferd15,16, 17,18,19,20,21,22,23,24,25, 26. Vi har brukt væske-celle TEM spore bevegelsen av personlige nanopartikler under løsemiddel fordamping, viser at bevegelsen av løsemiddel grensen er en viktig drivkraft for å indusere hydrogenion selvstendig montering på et substrat18 , 19. her, vi introdusere eksperimenter hvor vi kan observere prosessen med hydrogenion selvstendig montering med væske-celle TEM. Først vi tilbyr protokoller for syntese av platina og føre selen nanopartikler, før innføring av fabrikasjon prosedyrene for væske-celler for TEM og hvor å belaste nanopartikler i væske-cellen. Som representant resultater viser vi øyeblikksbilder fra TEM filmer av hydrogenion selvtillit forsamlingen drevet av løsemiddel tørking. Ved å spore individuelle partikler i disse filmene, kan vi forstå detaljert mekanismer for løsemiddel-tørking-mediert selvstendig montering på et enkelt hydrogenion nivå. Under selv-montering, følger platina nanopartikler silicon nitride vinduet bevegelsen av avdamping løsemiddel foran på grunn av sterk kapillære krefter opptrer på tynne løsemiddel lag. Lignende fenomener ble også observert for andre nanopartikler (bly selen) og underlag (silisium), indikerer at kapillær løsemiddel forsiden er en viktig faktor i partikkel migrasjon nær et substrat.

<table>
	<tbody>
		<tr>
			<td>ammonium hexachloroplatinate (IV)</td>
			<td>Sigma-Aldrich</td>
			<td>204021</td>
			<td></td>
		</tr>
		<tr>
			<td>ammonium tetrachloroplatinate (II)</td>
			<td>Sigma-Aldrich</td>
			<td>206105</td>
			<td></td>
		</tr>
		<tr>
			<td>tetramethylammonium bromide, 98%</td>
			<td>Sigma-Aldrich</td>
			<td>195758</td>
			<td></td>
		</tr>
		<tr>
			<td>poly(vinylpyrrolidone) powder</td>
			<td>Sigma-Aldrich</td>
			<td>234257</td>
			<td>Mw ~29,000</td>
		</tr>
		<tr>
			<td>ethylene glycol, anhydrous, 99.8%</td>
			<td>Sigma-Aldrich</td>
			<td>324558</td>
			<td></td>
		</tr>
		<tr>
			<td>n-hexane, anhydrous, 95%</td>
			<td>Samchun Chem.</td>
			<td>H0114</td>
			<td></td>
		</tr>
		<tr>
			<td>ethanol, anhydrous, 99.5%</td>
			<td>Sigma-Aldrich</td>
			<td>459836</td>
			<td></td>
		</tr>
		<tr>
			<td>oleylamine, 70%</td>
			<td>Sigma-Aldrich</td>
			<td>O7805</td>
			<td>Technical grade</td>
		</tr>
		<tr>
			<td>lead(II) acetate trihydrate, 99.99%</td>
			<td>Sigma-Aldrich</td>
			<td>467863</td>
			<td></td>
		</tr>
		<tr>
			<td>oleic acid, 90%</td>
			<td>Sigma-Aldrich</td>
			<td>364525</td>
			<td>Technical grade</td>
		</tr>
		<tr>
			<td>diphenyl ether, 99%</td>
			<td>Sigma-Aldrich</td>
			<td>P24101</td>
			<td>ReagentPlus</td>
		</tr>
		<tr>
			<td>selenium powder, 99.99%</td>
			<td>Sigma-Aldrich</td>
			<td>229865</td>
			<td></td>
		</tr>
		<tr>
			<td>tri-n-octylphosphine, 97%</td>
			<td>Strem</td>
			<td>15-6655</td>
			<td>Air sensistive</td>
		</tr>
		<tr>
			<td>Toluene, anhydrous, 99.9%</td>
			<td>Samchun Chem.</td>
			<td>T2419</td>
			<td></td>
		</tr>
		<tr>
			<td>acetone 99.8%</td>
			<td>Daejung Chem.</td>
			<td>1009-2304</td>
			<td></td>
		</tr>
		<tr>
			<td>potassium hydroxide, 95%</td>
			<td>Samchun Chem.</td>
			<td>P0925</td>
			<td></td>
		</tr>
		<tr>
			<td>p-type silicon-on-insulator wafers</td>
			<td>Soitec</td>
			<td>Power-SOI</td>
			<td>for liquid cells with silicon windows</td>
		</tr>
		<tr>
			<td>tetramethylammonium hydroxide, 25% in H2O</td>
			<td>J.T.Baker</td>
			<td>02-002-109</td>
			<td></td>
		</tr>
		<tr>
			<td>AZ 5214 E</td>
			<td>AZ Electronic Materials</td>
			<td>AZ 5214 E</td>
			<td>Positive photorest</td>
		</tr>
		<tr>
			<td>AZ-327</td>
			<td>AZ Electronic Materials</td>
			<td>AZ-327</td>
			<td>AZ 5214 develper</td>
		</tr>
		<tr>
			<td>indium pellets 99.98-99.99%</td>
			<td>Kurt J. Lesker Company</td>
			<td>EVMIN40EXEB</td>
			<td>thermal evaporator target</td>
		</tr>
		<tr>
			<td>1,2-dichlorobenzene, &#62;99%</td>
			<td>TCI</td>
			<td>D1116</td>
			<td></td>
		</tr>
		<tr>
			<td>pentadecane, &#62;99%</td>
			<td>Sigma-Aldrich</td>
			<td>P3406</td>
			<td></td>
		</tr>
		<tr>
			<td>buffered oxide etch 7:1</td>
			<td>microchemicals</td>
			<td>BOE 7-1 VLSI</td>
			<td></td>
		</tr>
		<tr>
			<td>phosphoric acid, 85%</td>
			<td>Samchun Chem.</td>
			<td>P0449</td>
			<td></td>
		</tr>
	</tbody>
</table>

liquid-cell transmission electron microscopy for tracking self-assembly of nanoparticles

Tørking en hydrogenion dispersjon er en allsidig måte å lage selv montert strukturer av nanopartikler, men mekanismen for denne prosessen er ikke fullt ut forstått. Vi har sporet baner av personlige nanopartikler bruker væske-celle overføring elektronmikroskop (TEM) for å undersøke mekanismen av monteringen. Her presenterer vi protokollene som brukes for væske-celle TEM studier av den selv-montering mekanismen. Først introduserer vi detaljerte syntetiske protokollene som brukes til å produsere jevnt størrelse platina og føre selen nanopartikler. Deretter presenterer vi de microfabrication prosessene som brukes til å produsere flytende celler med silicon nitride eller silisium windows og deretter beskriver lasting og imaging prosedyrer for væske-celle TEM teknikken. Flere notater er medregnet å skaffe nyttige tips for hele prosessen, inkludert å administrere vinduene skjøre cellen. Individuelle bevegelser nanopartikler sporet av væske-celle TEM avslørte at endringer i løsemiddel grensene skyldes fordampning berørt av selvtillit forsamlingen prosessen av nanopartikler. Løsemiddel grensene kjørte nanopartikler til primært skjemaet amorfe aggregat, etterfulgt av sammenslåing av aggregater å produsere en 2-dimensjonal (2D) selv samlet struktur. Disse atferd er også observert for ulike hydrogenion typer og annen væske-celle komposisjoner.

Væske-cellen består av en topp chip og en nederst chip, som er utstyrt med silicon nitride Vinduer gjennomsiktig til en elektronstråle med en tykkelse på 25 nm. Topp chip har et reservoar for lagring prøve løsningen og fordampet løsemiddel. Chips er gjort via konvensjonelle microfabrication behandling25. Maskene brukes for topp- og chips er vist i figur 1a og 1b, henholdsvis. <strong class="xfi...

Watch this Scientific Journal Video about Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles at JoVE.com

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles

Væske-celle overføring elektronmikroskop for sporing selvstendig montering av nanopartikler

Her introduserer vi eksperimentelle protokollene for sanntid observasjon av en selvstendig montering prosess med væske-celle overføring elektronmikroskop.

Drying a nanoparticle dispersion is a versatile way to create self-assembled structures of nanoparticles, but the mechanism of this process is not fully ...

Research

JoVE Journal

Chemistry

Origami Inspired Self-assembly of Patterned and Reconfigurable Particles

Origami Inspirert Self-montering av mønstret og rekonfigurerbar Partikler

There are numerous techniques such as photolithography, electron-beam lithography and soft-lithography that can be used to precisely pattern two ...

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Dannelse av Bestilte biomolekylære strukturer ved selvbygging av Short Peptider

In nature, complex functional structures are formed by the self-assembly of biomolecules under mild conditions. Understanding the forces that control ...

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

Selvbygging av komplekse Todimensjonale figurer fra Single DNA Fliser

Current methods in DNA nano-architecture have successfully engineered a variety of 2D and 3D structures using principles of self-assembly. In this ...

Obtaining 3D Chemical Maps by Energy Filtered Transmission Electron Microscopy Tomography

Få 3D kjemiske kart av energi filtrert overføring elektronmikroskop tomografi

Energy filtered transmission electron microscopy tomography (EFTEM tomography) can provide three-dimensional (3D) chemical maps of materials at a ...

Analysis of Minerals Produced by hFOB 1.19 and Saos-2 Cells Using Transmission Electron Microscopy with Energy Dispersive X-ray Microanalysis

Analyse av mineraler produsert av hFOB 1,19 og Saos-2 celler ved hjelp av overføring elektronmikroskop med energi dispersiv X-ray Microanalysis

This video presents the use of transmission electron microscopy with energy dispersive X-ray microanalysis (TEM-EDX) to compare the state of minerals in ...

Using Graphene Liquid Cell Transmission Electron Microscopy to Study in Situ Nanocrystal Etching

Using Graphene Liquid Cell Transmission Electron Microscopy to Study in Situ Nanocrystal Etching

Bruk av Graphene væske celle overføring elektronmikroskop in Situ Nanocrystal etsning

Graphene liquid cell electron microscopy provides the ability to observe nanoscale chemical transformations and dynamics as the reactions are occurring in ...

Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

Utføre spektroskopi på Plasmonic nanopartikler med Transmission-baserte Nomarski-type differensial interferens kontrast mikroskopi

Differential interference contrast (DIC) microscopy is a powerful imaging tool that is most commonly employed for imaging microscale objects using ...

Preparation of Graphene-Supported Microwell Liquid Cells for In Situ Transmission Electron Microscopy

Preparation of Graphene-Supported Microwell Liquid Cells for In Situ Transmission Electron Microscopy

Utarbeidelse av grafen-støttede Mikrotiterplateleser væske celler for in situ Transmission Electron mikroskopi

The fabrication and preparation of graphene-supported microwell liquid cells (GSMLCs) for in situ electron microscopy is presented in a stepwise protocol. ...

Synthesis of Monodisperse Cylindrical Nanoparticles via Crystallization-driven Self-assembly of Biodegradable Block Copolymers

Syntese av Monodisperse sylindriske nanopartikler via krystallisering-drevet selv montering av biologisk nedbrytbart Block Kopolymerer

The production of monodisperse cylindrical micelles is a significant challenge in polymer chemistry. Most cylindrical constructs formed from diblock ...

Self-Assembly of Hybrid Lipid Membranes Doped with Hydrophobic Organic Molecules at the Water/Air Interface

Selvmontering av hybrid lipidmembraner dopet med hydrofobe organiske molekyler ved vann-/luftgrensesnittet

Because of their unique properties, including an ultrathin thickness (3-4 nm), ultrahigh resistivity, fluidity and self-assembly ability, lipid bilayers ...