Name: long-term live imaging device for improved experimental manipulation of zebrafish larvae
Uploaded: 2017-10-27T15:00:00
Description: Watch this Scientific Journal Video about Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae at JoVE.com

Forfatterne vil gerne anerkende primære projektfinansiering fra det Morgridge Institut for forskning og laboratoriet for optisk og beregningsmæssige Instrumentation. Vi anerkender også midler fra NIH # R01GM102924 (AH og KWE). KH, JMS, RS, AH og KWE udtænkt og designet af undersøgelsen. KH og JMS udførte alle eksperimenter med støtte fra DL, KP og RS. KH, JS, RS, AH og KWE bidraget til skrivning af håndskriftet.

Bemærk: base zWEDGI design blev formuleret for zebrafisk larver, der er 2-4 dage efter befrugtning (dpf) og følge retningslinjerne fra University of Wisconsin-Madison forskning dyr Ressourcecenter.
 1. design og 3D udskrivning af forme <ol><li>Model PDMS komponent af enheden med ønskede geometrier og attributter i et 3D-modellering software 5. Oprette en forsamling af en tom mold og PDMS del og generere en negative støbeform til PDMS del ved at skabe et hulrum i...

<ol>
	<li>Yoo, S. K., Freisinger, C. M., LeBert, D. C., Huttenlocher, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Early+redox,+Src+family+kinase,+and+calcium+signaling+integrate+wound+responses+and+tissue+regeneration+in+zebrafish.">Early redox, Src family kinase, and calcium signaling integrate wound responses and tissue regeneration in zebrafish.</a> J. Cell Biology. 199 (2), 225-234 (2012).</li><li>Kawakami, A., Fukazawa, T., Takeda, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Early+fin+primordia+of+zebrafish+larvae+regenerate+by+a+similar+growth+control+mechanism+with+adult+regeneration.">Early fin primordia of zebrafish larvae regenerate by a similar growth control mechanism with adult regeneration.</a> Dev. Dynam. 231 (4), 693-699 (2004).</li><li>Konantz, J., Antos, C. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reverse+genetic+morpholino+approach+using+cardiac+ventricular+injection+to+transfect+multiple+difficult-to-target+tissues+in+the+zebrafish+larva.">Reverse genetic morpholino approach using cardiac ventricular injection to transfect multiple difficult-to-target tissues in the zebrafish larva.</a> JoVE. (88), (2014).</li><li>Hall, C., Flores, M. F., Kamei, M., Crosier, K., Crosier, P., Sampath, K., Roy, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Live+Imaging+Innate+Immune+Cell+Behavior+During+Normal+Development,+Wound+Healing+and+Infection.">Live Imaging Innate Immune Cell Behavior During Normal Development, Wound Healing and Infection.</a> Live Imaging in Zebrafish: Insights into Development and Disease. , (2010).</li><li>Huemer, K., Squirrell, J. M., Swader, R., LeBert, D. C., Huttenlocher, A., Eliceiri, K. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=zWEDGI:+Wounding+and+Entrapment+Device+for+Imaging+Live+Zebrafish+Larvae.">zWEDGI: Wounding and Entrapment Device for Imaging Live Zebrafish Larvae.</a> Zebrafish. , (2016).</li><li>Lisse, T. S., Brochu, E. A., Rieger, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Capturing+tissue+repair+in+zebrafish+larvae+with+time-lapse+brightfield+stereomicroscopy.">Capturing tissue repair in zebrafish larvae with time-lapse brightfield stereomicroscopy.</a> JoVE. (95), (2015).</li><li>Kamei, M., Isogai, S., Pan, W., Weinstein, B. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Imaging+blood+vessels+in+the+zebrafish.">Imaging blood vessels in the zebrafish.</a> Methods Cell Biol. 100, 27-54 (2010).</li><li>Graeden, E., Sive, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Live+imaging+of+the+zebrafish+embryonic+brain+by+confocal+microscopy.">Live imaging of the zebrafish embryonic brain by confocal microscopy.</a> JoVE. (26), (2009).</li><li>Petzold, A. M., Bedell, V. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=SCORE+imaging:+specimen+in+a+corrected+optical+rotational+enclosure.">SCORE imaging: specimen in a corrected optical rotational enclosure.</a> Zebrafish. 7 (2), 149-154 (2010).</li><li>Macdonald, N. P., Zhu, F., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessment+of+biocompatibility+of+3D+printed+photopolymers+using+zebrafish+embryo+toxicity+assays.">Assessment of biocompatibility of 3D printed photopolymers using zebrafish embryo toxicity assays.</a> Lab Chip. 16 (2), 291-297 (2016).</li><li>LeBert, D. C., Squirrell, J. M., Huttenlocher, A., Eliceiri, K. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Second+harmonic+generation+microscopy+in+zebrafish.">Second harmonic generation microscopy in zebrafish.</a> Methods Cell Biol. 133, 55-68 (2016).</li><li>White, R. M., Sessa, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transparent+Adult+Zebrafish+as+a+Tool+for+In+Vivo+Transplantation+Analysis.">Transparent Adult Zebrafish as a Tool for In Vivo Transplantation Analysis.</a> Cell Stem Cell. 2 (2), 183-189 (2008).</li><li>LeBert, D. C., Squirrell, J. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Matrix+metalloproteinase+9+modulates+collagen+matrices+and+wound+repair.">Matrix metalloproteinase 9 modulates collagen matrices and wound repair.</a> Development. 142 (12), 2136-2146 (2015).</li><li>Campagnola, P. J., Millard, A. C., Terasaki, M., Hoppe, P. E., Malone, C. J., Mohler, W. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Three-dimensional+high-resolution+second-harmonic+generation+imaging+of+endogenous+structural+proteins+in+biological+tissues.">Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues.</a> Biophys. J. 82 (1 Pt 1), 493-508 (2002).</li><li>Schindelin, J., Arganda-Carreras, I., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Fiji:+an+open-source+platform+for+biological-image+analysis.">Fiji: an open-source platform for biological-image analysis.</a> Nat. Methods. 9 (7), 676-682 (2012).</li></ol>

Formålet med zWEDGI enheden er at fange 3D tid bortfalder imaging af stabiliserende og orientere fisk inden for en høj opløsning mikroskop mål lille arbejde afstand. Mens opfylder disse specifikationer, er det også en forbedring over traditionelle agar-baserede forberedelse til live imaging. Der er tre vigtige trin (nedenfor) i fabrikation af zWEDGI, som, hvis det ikke gøres korrekt, kan resultere i defekte enheder:
PDMS forberedelse (figur 5A)
<p class=...

Den regenerative kapacitet af zebrafisk larver Danio rerio gøre dem en ideel model organisme behandlingen af sår svar samt healing og genvækst1,2,3,4. Adgang til en bred vifte af transgene zebrafisk linjer og zebrafisks anatomiske gennemsigtighed yderligere forbedre deres nytte for i vivo studier af sår svar events samt langsigtede regenerative processer4. Undersøgelse af disse biologiske processer med høj opløsning time-lapse Fluorescens mikroskopi derfor kræver en live imaging zebrafisk enhed, der giver mulighed for høj stabilitet og minimal bevægelse af zebrafisk larve samtidig opretholde levedygtighed. Det er afgørende at enheden giver mulighed for effektiv sårede mens healing og regeneration forekomme upåvirket af enheden.
Live imaging stabilisering standardmetoden til indlejring af larve i Agarosen under live imaging begrænser vækst og sår regenerering5 og kan øge dødelighed, da larverne begynder at vise tegn på hjertesygdomme stress og vævet nekrose efter fire timer4. Derfor, fjernelse af Agarosen fra områder af interesse er ofte nødvendigt at tillade normal udvikling og regeneration6, udsætte larverne til potentielle skader som Agarosen er skåret væk. Derudover skal brugeren med Agarosen indlejring teknik, orientere larver i kort tid før Agarosen størkner5,6,7. Hurtigt manipulere larver ikke kun kræver dygtighed af brugeren, der også risiko for skader på larve. Selv om metoder til at stabilisere larve til live imaging er blevet beskrevet til at omgå disse ulemper, såsom ridged agar wells3 eller puder8, brug af silikone vakuum fedt til at skabe en billeddannelse kammer med PVC rør eller andre materialer6og roterende slanger9, mange af disse metoder er labor intensiv, rodet, ofte ikke-genanvendelig og tillader ikke for miljømæssige manipulation (narkotika behandlinger, såret osv.) efter fiskene er blevet monteret.
Derfor, zWEDGI enheden (figur 1) var designet til at overvinde nogle af ulemperne af agar montering for langsigtet live imaging af zebrafisk larver samtidig tillade manipulation af prøven. ZWEDGI består af tre semi-åbne opdelte kamre (figur 1A) for at give mulighed for lastning, tilbageholdenhed, sårede og billeddannelse af 2-4 dage efter befrugtningen zebrafisk larver. Enheden er fabrikeret fra Polydimethylsiloxan (PDMS) og placeret på cover slip af en 60 mm glas bund imaging parabol. Design præsenteres her var beregnet til sår healing undersøgelser, men brugen af et modulært design og standard fabrikation teknologi gøre zWEDGI designet modificerbare og imødekommenhed over for en række eksperimentelle procedurer, især for procedurer, kræve minimal tilbageholdenhed med eksperimentelle manipulation og langsigtede billeddannelse.

<table border="0" cellpadding="0" cellspacing="0" width="920">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:421px;">Fabricate molds</td>
			<td style="width:127px;"></td>
			<td style="width:145px;"></td>
			<td style="width:227px;"></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Solidworks Professional Accedemic Research 3D modeling software</td>
			<td>Dassault Systemes</td>
			<td>SPX0117-01</td>
			<td>Fisher Unitech</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Viper Si2 SLA 3D printer</td>
			<td>3D Systems Inc.</td>
			<td>23200-902</td>
			<td>3D Systems Inc.</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Accura 60 photopolymer resin</td>
			<td>3D Systems Inc.</td>
			<td>24075-902</td>
			<td>3D Systems Inc.</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">denatured alcohol</td>
			<td>Sunnyside</td>
			<td>5613735</td>
			<td>Menards</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">UV post cure apparatus</td>
			<td>3D Systems Inc.</td>
			<td>23363-101-00</td>
			<td>3D Systems Inc.</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">TouchNTuff nitrile gloves</td>
			<td>Ansell</td>
			<td>92-600</td>
			<td>McMaster Carr</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">220B, 400B, 600 grit T414 blue-bak sandpaper&#160;</td>
			<td>Norton</td>
			<td>66261139359, 54, 52</td>
			<td>MSC</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">borosilicate glass disc, 2&#34; diameter</td>
			<td>McMaster-Carr</td>
			<td>MIL-G-47033</td>
			<td>McMaster-Carr</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">ultrasonicator cleaner</td>
			<td>Branson</td>
			<td>1510R-MTH</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">isopropyl rubbing alcohol 70%</td>
			<td>Hydrox</td>
			<td>54845T43</td>
			<td>McMaster-Carr</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">10oz clear plastic cup</td>
			<td>WNA Masterpiece</td>
			<td>557405</td>
			<td>Amazon</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">6&#34;craft stick</td>
			<td>Perfect Stix</td>
			<td>Craft WTD-500</td>
			<td>Amazon</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Name</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Fabricate zWEDGI PDMS device</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Sylgard 184 silicon elastomeric kit&#160;</td>
			<td>Dow-Corning</td>
			<td>4019862</td>
			<td>Ellworth Adhesives&#160;</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">10mL syringe</td>
			<td>Becton Dickinson</td>
			<td>305219</td>
			<td>Vitality Medical Inc</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">desiccator</td>
			<td>Bel-Art Scienceware</td>
			<td>F42027-0000</td>
			<td>Amazon</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">4 in ratcheting bar clamp</td>
			<td>Pittsburgh</td>
			<td>68974</td>
			<td>Harbor Freight</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">lab oven</td>
			<td>Quincy Lab Inc.</td>
			<td>20GC</td>
			<td>Global Industrial</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">tweezer set</td>
			<td>Aven</td>
			<td>549825</td>
			<td>McMaster-Carr</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">compressed air filtered nozzle</td>
			<td>Innotech</td>
			<td>TA-N2-2000FT</td>
			<td>Cleanroom Supply</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">vacuum bench vise</td>
			<td>Wilton Tool Group</td>
			<td>63500</td>
			<td>MSC Industrial</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">55mm glass bottom dish; 30mm micro-well #1.5 cover glass</td>
			<td>Cellvis</td>
			<td>D60-30-1.5-N</td>
			<td>Cellvis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">plasma cleaner</td>
			<td>Harrick Plasma</td>
			<td>PDC-001</td>
			<td>Harrick Plasma</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Name</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Loading Larvae</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Pipetteman, P200</td>
			<td>Gilson</td>
			<td>F123601</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">100% ethanol (diluted to 70% with water prior to use)</td>
			<td>Pharmco-aaper</td>
			<td>111000200</td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Transfer pipette</td>
			<td>Fisherbrand</td>
			<td>13-711-5A</td>
			<td>Fisher Scientific</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">powdered skim milk</td>
			<td></td>
			<td>2902887</td>
			<td>MP Biomedicals</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">double distilled water</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">N-phenylthiorurea</td>
			<td>Sigma-Aldrich</td>
			<td>P7629</td>
			<td>Sigma-Aldrich</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">tricaine (ethyl 3-aminobenzoate)</td>
			<td></td>
			<td>C-FINQ-UE</td>
			<td>Western Chemical</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">low melting point agarose</td>
			<td>Sigma-Aldrich</td>
			<td>A0701</td>
			<td>Sigma-Aldrich</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">heat block (dry bath incubator)</td>
			<td>Fisher Scientific</td>
			<td>11-718-2</td>
			<td>Fisher Scientific</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">E3 buffer&#160;</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">large orifice pipette tip, 200 uL</td>
			<td>Fisherbrand</td>
			<td>02-707-134</td>
			<td>Fisher Scientific</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">General purpose pipette tip, 200 uL</td>
			<td>Fisherbrand</td>
			<td>21-197-8E</td>
			<td>Fisher Scientific</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">#15 scalpel blade&#160;</td>
			<td>Feather</td>
			<td>2976</td>
			<td>Amazon</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">25G syringe needle</td>
			<td>BD&#160;</td>
			<td>BD305122</td>
			<td>Fisher Scientific</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Name</td>
			<td>Company</td>
			<td>Catalog Number</td>
			<td>Comments</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Imaging</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">inverted microscope</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;">Imaris imaging software</td>
			<td>Bitplane</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

long-term live imaging device for improved experimental manipulation of zebrafish larvae

Zebrafisk larve er en vigtig model organisme for både udviklingsmæssige biologi og sårheling. Desuden er zebrafisk larve et værdifuldt system til live høj opløsning mikroskopisk billeddannelse af dynamiske biologiske fænomener i tid og rum med cellulære opløsning. Men den traditionelle metode til Agarosen indkapsling af live imaging kan hindre larve udvikling og væv genvækst. Derfor dette manuskript beskriver zWEDGI (zebrafisk Wounding og Entrapment enhed for vækst og Imaging), som blev designet og fremstillet som et funktionelt opdelte enhed til at orientere larver til høj opløsning mikroskopi samtidig tillader det halefinnen er transection inden for enheden og efterfølgende uhæmmet hale udvikling og re-vækst. Denne enhed tillader sårede og langsigtede imaging samtidig opretholde levedygtighed. Formen zWEDGI er 3D udskrives, i customizability af dens geometrier gør det let modificeret til forskellige zebrafisk billedbehandlingsprogrammer. Desuden, zWEDGI tilbyder mange fordele, såsom adgang til larve under eksperimenter for at have såret eller for anvendelsen af reagenser, sideløbende orientering af flere larver for strømlinet imaging og genbrugelighed af enheden.

ZWEDGI PDMS mikrofluid enhed er et funktionelt opdelte enhed designet til at rumme fire hovedfunktioner (nedenfor) forbundet med live imaging af halefinnen såret healing og genvækst i zebrafisk larver. PDMS blev valgt til zWEDGI fabrikation, fordi det ikke kun er let tilgængelige og en industristandard for biokompatibilitet, men også fungerer godt i forme. Derudover gør PDMS enheden genbruges og blottet for hårde eller skarpe kanter når enheden er dannet. ZWEDGI specifikt tillader ...

Watch this Scientific Journal Video about Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae at JoVE.com

Long-term Live Imaging Device for Improved Experimental Manipulation of Zebrafish Larvae

Dette manuskript beskriver zWEDGI (zebrafisk Wounding og Entrapment enhed for vækst og Imaging), som er en opdelte enhed designet til at orientere og begrænse zebrafisk larver. Design tillader hale transection og langsigtede samling af høj opløsning fluorescerende mikroskopi billeder af sårheling og regenerering.

Langsigtet Live Imaging enhed for forbedret eksperimentelle manipulering af zebrafisk larver

The zebrafish larva is an important model organism for both developmental biology and wound healing. Further, the zebrafish larva is a valuable system for ...

Research

JoVE Journal

Bioengineering

Time-lapse Fluorescence Imaging of Arabidopsis Root Growth with Rapid Manipulation of The Root Environment Using The RootChip

Time-lapse fluorescensimagografi af Arabidopsis rodvækst med Rapid Manipulation af The Root miljøet ved hjælp af RootChip

The root functions as the physical anchor of the plant and is the organ responsible for uptake of water and mineral nutrients such as nitrogen, ...

Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae

Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae

Brug mikrofluidik Chips til Live Imaging og Undersøgelse af Injury Responses i<em&gt; Drosophila</em&gt; Larver

Live imaging is an important technique for studying cell biological processes, however this can be challenging in live animals. The translucent cuticle of ...

Long-term Intravital Immunofluorescence Imaging of Tissue Matrix Components with Epifluorescence and Two-photon Microscopy

Langsigtet Intravital Immunofluorescens Imaging af Tissue Matrix Komponenter med epifluorescens og to-foton mikroskopi

Besides being a physical scaffold to maintain tissue morphology, the extracellular matrix (ECM) is actively involved in regulating cell and tissue ...

High-resolution Spatiotemporal Analysis of Receptor Dynamics by Single-molecule Fluorescence Microscopy

Høj opløsning Spatiotemporal Analyse af receptor Dynamics ved Single-molekyle fluorescensmikroskopi

Single-molecule microscopy is emerging as a powerful approach to analyze the behavior of signaling molecules, in particular concerning those aspect (e.g., ...

The Multi-organ Chip - A Microfluidic Platform for Long-term Multi-tissue Coculture

Multi-orgel Chip - En Mikrofluid platform for langsigtet Multi-væv cokultur

The ever growing amount of new substances released onto the market and the limited predictability of current in vitro test systems has led to a high need ...

Live Cell Imaging during Mechanical Stretch

Live-Cell Imaging under Mechanical Stretch

There is currently a significant interest in understanding how cells and tissues respond to mechanical stimuli, but current approaches are limited in ...

Hybrid &#181;CT-FMT imaging and image analysis

Hybrid Ãƒâ€šÃ‚ÂµCT-FMT imaging and image analysis

Hybrid μCT-FMT billedbehandling og billedanalyse

Fluorescence-mediated tomography (FMT) enables longitudinal and quantitative determination of the fluorescence distribution in vivo and can be used to ...

High-resolution Imaging of Nuclear Dynamics in Live Cells under Uniaxial Tensile Strain

High-Resolution Imaging af nuklear dynamik i levende celler under Uniaxial træk stamme

Extracellular mechanical strain is known to elicit cell phenotypic responses and has physiological relevance in several tissue systems. To capture the ...

Targeting the Rat's Small Bowel: Long-Term Infusion into the Superior Mesenteric Artery

Målretning af rottens lille tarm: Langvarig infusion i superior mesenterisk arterie

The superior mesenteric artery can be cannulated in humans through minimally invasive radiological catheterization of the femoral or axillary artery. SMA ...

Advanced 3D Liver Models for In vitro Genotoxicity Testing Following Long-Term Nanomaterial Exposure

Avancerede 3D-levermodeller til in vitro genotoksicitetstest efter langvarig nanomaterialeeksponering

Due to the rapid development and implementation of a diverse array of engineered nanomaterials (ENM), exposure to ENM is inevitable and the development of ...