Generation of MDA-MB-231 Cells Stably Expressing Histone H2B-mCherry Using Lentiviral Vectors
4:00
Synchronization of Cells Stably Expressing H2B-mCherry
6:02
Incorporation of the Synchronized Cells into Collagen I Matrices
7:18
Live Cell Imaging and Matrix Deformation During Cell Division in 3D Collagen Matrices
8:46
Results: Insights from Imaging Mammalian Cell Division in 3D Collagen Matrices
9:45
Conclusion
Transcript
The overall goal of this procedure is to study mammalian cell division and cell matrix interactions in a physiologically relevant 3D environment. The main advantage of this technique is that it provides an efficient and general approach to study m
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Denne protokollen effektivt studier pattedyr celledeling i 3D kollagen matriser ved å integrere synkronisering av cellen divisjon, overvåking av løpene i 3D matriser med live-celle tenkelig teknikk, tid-løst AC confocal refleksjon mikroskopi og kvantitativ tenkelig analyse.