Generation of MDA-MB-231 Cells Stably Expressing Histone H2B-mCherry Using Lentiviral Vectors
4:00
Synchronization of Cells Stably Expressing H2B-mCherry
6:02
Incorporation of the Synchronized Cells into Collagen I Matrices
7:18
Live Cell Imaging and Matrix Deformation During Cell Division in 3D Collagen Matrices
8:46
Results: Insights from Imaging Mammalian Cell Division in 3D Collagen Matrices
9:45
Conclusion
Transcript
The overall goal of this procedure is to study mammalian cell division and cell matrix interactions in a physiologically relevant 3D environment. The main advantage of this technique is that it provides an efficient and general approach to study m
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Este protocolo eficientemente estudios División de célula mamífera en matrices de colágeno 3D mediante la integración de sincronización de la división celular, monitoreo de eventos de la división de matrices 3D utilizando la técnica de imágenes de células vivas, la microscopia confocal tiempo-resolved de la reflexión cuantitativos y análisis de imágenes.