Generation of MDA-MB-231 Cells Stably Expressing Histone H2B-mCherry Using Lentiviral Vectors
4:00
Synchronization of Cells Stably Expressing H2B-mCherry
6:02
Incorporation of the Synchronized Cells into Collagen I Matrices
7:18
Live Cell Imaging and Matrix Deformation During Cell Division in 3D Collagen Matrices
8:46
Results: Insights from Imaging Mammalian Cell Division in 3D Collagen Matrices
9:45
Conclusion
Transcript
The overall goal of this procedure is to study mammalian cell division and cell matrix interactions in a physiologically relevant 3D environment. The main advantage of this technique is that it provides an efficient and general approach to study m
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Bu iletişim kuralı verimli çalışmalar 3D kollajen matrisler memeli hücre bölünmesi hücre bölünmesi, eşitlenmesi entegre ederek 3B matrisleri canlı hücre görüntüleme tekniği, confocal yansıması zaman çözüldü mikroskobu kullanarak bölümü olayların izlenmesi ve nicel analiz görüntüleme.