Name: autologous microfractured and purified adipose tissue for arthroscopic management of osteochondral lesions of the talus
Uploaded: 2018-01-23T13:00:00
Description: Watch this Scientific Journal Video about Autologous Microfractured and Purified Adipose Tissue for Arthroscopic Management of Osteochondral Lesions of the Talus at JoVE.com

प्रक्रियाओं Lipogems सिस्टम का उपयोग कर किया जाता है ।

सभी मानव प्रतिभागियों को शामिल अध्ययन में प्रदर्शन प्रक्रियाओं संस्थागत और/या राष्ट्रीय अनुसंधान समिति के नैतिक मानकों के अनुसार थे, और १९६४ हेलसिंकी घोषणा और उसके बाद के संशोधन या तुलनीय नैतिक के स?...

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हाल के वर्षों में, नैदानिक और नैदानिक परीक्षणों ADSCs के प्रभाव पर अपना ध्यान केंद्रित किया है कि विभिन्न गठिया विकृतियों का इलाज. इस लेख का उद्देश्य आर्थ्रोस्कोपिक microperforations के सहयोग से microfractured और शुद्ध वसा ऊ?...

आर्थोस्कोपी तैल्य (OLTs) के osteochondral घावों के उपचार के लिए सोने के मानक है दर्द से राहत के उद्देश्य से, कार्यक्षमता बहाल करने, और जीवन की गुणवत्ता में सुधार, विशेष रूप से युवा और सक्रिय रोगियों में ।
वर्तमान में, आर्थ्रोस्कोपिक तकनीक तीन तरीकों से वर्गीकृत किया जा सकता है । प्रतिकारक तकनीक अस्थि मज्जा से एक दुल्हन और घावों के स्तर पर microperforations के माध्यम से व्युत्पंन कोशिकाओं को उत्तेजित करता है । पुनर्निर्माण तकनीक एक ऑटोलॉगस या heterologous ostechondral भ्रष्टाचार का उपयोग कर घाव की जगह । reअपक्षय तकनीक multipotent कोशिकाओं की क्षमता में अंतर करने के लिए और क्षतिग्रस्त ऊतक पुनर्निर्माण करने के लिए दोहराने के लिए1,2,3,4,5,6 .
हाल के वर्षों में, अपक्षयी तकनीक कई विट्रो में और vivo अध्ययनों में OLTs के उपचार के लिए विषय किया गया है, और विशेष रूप से mesenchymal स्टेम वसा ऊतक (ADSCs) से व्युत्पंन कोशिकाओं7,8 , 9. ये mesenchymal स्टेम कोशिकाएं अन्य multipotent कोशिकाओं के समान रूपात्मक और कार्यात्मक विशेषताओं का प्रदर्शन, अन्य ऊतकों से पृथक; वे भी कई और अलग सेलुलर लाइनों दोनों विट्रो में और vivo10,11,12,13में अंतर करने की क्षमता है। इन कोशिकाओं के बारे में अनुसंधान पर ध्यान केंद्रित मुख्य रूप से उनके स्थानीयकरण की वजह से है, वास्तव में वे ५,००० ऊतक के ग्राम प्रति कोशिकाओं की एकाग्रता के साथ सामान्य शरीर के वजन के 30% करने के लिए 10% से प्रतिनिधित्व करते हैं13,14. दूसरी ओर, एक कारक है कि इन कोशिकाओं के उपयोग की सीमा प्रयोगशाला प्रक्रियाओं के दौरान उनके हैंडलिंग से संबंधित है । adipocytes, कोलेजन फाइबर के समुच्चय युक्त lipoaspirate, और सामांय संवहनी घटकों कोलेजन एक प्रकार मैं के साथ संसाधित enzymatically है, और संस्कृति से पहले hemolysis के अधीन । यहां उद्देश्य microfractured और शुद्ध वसा ऊतक का उपयोग कर तैल्य के osteochondral घावों के उपचार के लिए प्रोटोकॉल का वर्णन है ।

<table>
	<tbody>
		<tr>
			<td>PROCESS KIT - PROCESSING KIT FOR FAT TISSUE</td>
			<td>LIPOGEMS</td>
			<td>LG PK 60</td>
			<td>Lipogems Kit to obtain microfractured and purified ADSCs</td>
		</tr>
		<tr>
			<td>HINTERMANN SPREADER</td>
			<td>INTEGRA</td>
			<td>119654</td>
			<td>The spreader allow to access most of the talar dome, in particular in case of posterior lesion</td>
		</tr>
		<tr>
			<td>CUP CURETTE</td>
			<td>ARTHREX</td>
			<td>AR-8655-02</td>
			<td>To remove the damaged cartilage and necrotic and sclerotic bone</td>
		</tr>
		<tr>
			<td>CHONDRAL PICK 30&#176; TIP / 60&#176; TIP</td>
			<td>ARTHREX</td>
			<td>AR-8655-05 
			AR-8655-06</td>
			<td>To perfrom microperforation at the level of the lesion, stimulating bleeding from the subchondral bone</td>
		</tr>
		<tr>
			<td>SHAVER</td>
			<td>ARTHREX</td>
			<td>AR-7300SR</td>
			<td>To clean the joint and aspirate water</td>
		</tr>
	</tbody>
</table>

autologous microfractured and purified adipose tissue for arthroscopic management of osteochondral lesions of the talus

हाल के वर्षों में, अपक्षयी तकनीक तेजी से अध्ययन किया गया है और तैल्य के osteochondral घावों के इलाज के लिए इस्तेमाल किया । विशेष रूप से, कई अध्ययनों से वसा ऊतक से व्युत्पंन mesenchymal स्टेम कोशिकाओं पर अपना ध्यान केंद्रित किया है । वसा-व्युत्पन्न स्टेम सेल (ADSCs) रूपात्मक विशेषताओं और अन्य mesenchymal कोशिकाओं के समान गुणों का प्रदर्शन, और कई सेलुलर लाइनों में अंतर करने में सक्षम हैं. इसके अलावा, इन कोशिकाओं को भी व्यापक रूप से चमड़े के नीचे ऊतक में उपलब्ध हैं, सामांय शरीर के वजन का 10-30% का प्रतिनिधित्व, ऊतक के ग्राम प्रति ५,००० कोशिकाओं की एकाग्रता के साथ ।
प्रस्तुत तकनीक में, पहला कदम पेट से कटाई ADSCs और microfracture और शुद्धि की प्रक्रिया शामिल है; अगले, शल्य प्रक्रिया पूरी तरह से arthroscopically, कम नरम ऊतक विच्छेदन, बेहतर संयुक्त दृश्य के साथ प्रदर्शन किया है, और एक तेजी से वसूली मानक खुली प्रक्रियाओं के साथ तुलना में । आर्थोस्कोपी एक पहले चरण की विशेषता है जिसमें घाव की पहचान, पृथक, और microperforations के साथ तैयार की गई है; दूसरा कदम, सूखी प्रदर्शन, घाव के स्तर पर वसा ऊतक के इंजेक्शन शामिल है ।
जनवरी २०१६ और २०१६ सितंबर के बीच, चार रोगियों microfractured और शुद्ध वसा ऊतक के साथ तैल्य के osteochondral घावों के आर्थ्रोस्कोपिक उपचार से गुजरा । सभी रोगियों कोई रिपोर्ट जटिलताओं के साथ सर्जरी के बाद छह महीने नैदानिक सुधार की सूचना दी । नवीनतम अनुवर्ती में कार्यात्मक स्कोर को प्रोत्साहित कर रहे है और पुष्टि करते है कि तकनीक विश्वसनीय दर्द राहत और तैल्य के osteochondral घावों के साथ रोगियों में सुधार प्रदान करता है ।

जनवरी २०१६ और २०१६ सितंबर के बीच, चार रोगियों microfractured और शुद्ध वसा ऊतक के साथ तैल्य के osteochondral घावों के आर्थ्रोस्कोपिक उपचार से गुजरा । सभी रोगियों सर्जरी के बाद छह महीने नैदानिक सुधार की सूचना ?...

Watch this Scientific Journal Video about Autologous Microfractured and Purified Adipose Tissue for Arthroscopic Management of Osteochondral Lesions of the Talus at JoVE.com

Autologous Microfractured and Purified Adipose Tissue for Arthroscopic Management of Osteochondral Lesions of the Talus

इस अध्ययन का उद्देश्य microfractured और शुद्ध वसा-व्युत्पन्न स्टेम कोशिकाओं का उपयोग कर तैल्य के osteochondral घावों के आर्थ्रोस्कोपिक उपचार के लिए एक प्रोटोकॉल की रिपोर्ट करने के लिए है ।

Osteochondral के तैल्य घावों के आर्थ्रोस्कोपिक प्रबंधन के लिए ऑटोलॉगस Microfractured और शुद्ध वसा ऊतक

In recent years, regenerative techniques have been increasingly studied and used to treat osteochondral lesions of the talus. In particular, several ...

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