Selecting Single Cell Clones and Characterizing MeCP2 Reporter Expression
4:31
Screening the Library for Reactivation Using Hygromycin B Selection
6:44
Validating the Identified Hairpins ( Hits )
9:27
Results: Isolation of Clones with the Reporter on the Inactive X Chromosome and Characterization of Hygromycin Resistance
10:17
Conclusion
Transcript
Begin this procedure with preparation of mouse tendon fibroblasts, as described in the text protocol. Harvest the cells from a 10-centimeter plate by first aspirating the media. Then add one milliliter of 0.05%trypsin EDTA and incubate for two to
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我们报告一个小发夹 RNA (shRNA) 和下一代测序协议, 以确定在小鼠细胞系的 X 染色体失活的监管者与萤火虫荧光素酶和潮霉素抵抗基因融合到甲基 CpG 结合蛋白 2 (MeCP2)非活性 X 染色体上的基因。