Selecting Single Cell Clones and Characterizing MeCP2 Reporter Expression
4:31
Screening the Library for Reactivation Using Hygromycin B Selection
6:44
Validating the Identified Hairpins ( Hits )
9:27
Results: Isolation of Clones with the Reporter on the Inactive X Chromosome and Characterization of Hygromycin Resistance
10:17
Conclusion
Transcript
Begin this procedure with preparation of mouse tendon fibroblasts, as described in the text protocol. Harvest the cells from a 10-centimeter plate by first aspirating the media. Then add one milliliter of 0.05%trypsin EDTA and incubate for two to
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Vi rapporterer en lille hårnål RNA (shRNA) og næste generation sequencing-baseret protokol til at identificere regulatorer af x-kromosom Inaktiveringen i en murine cellelinie med firefly luciferase og hygromycin resistensgener smeltet til methyl CpG bindende protein 2) MeCP2) gen på det inaktive X kromosom.