Selecting Single Cell Clones and Characterizing MeCP2 Reporter Expression
4:31
Screening the Library for Reactivation Using Hygromycin B Selection
6:44
Validating the Identified Hairpins ( Hits )
9:27
Results: Isolation of Clones with the Reporter on the Inactive X Chromosome and Characterization of Hygromycin Resistance
10:17
Conclusion
Transcript
Begin this procedure with preparation of mouse tendon fibroblasts, as described in the text protocol. Harvest the cells from a 10-centimeter plate by first aspirating the media. Then add one milliliter of 0.05%trypsin EDTA and incubate for two to
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우리 보고 작은 헤어핀 RNA (shRNA) 반딧불 luciferase murine 셀 라인에서 x 염색체 비활성화의 레 귤 레이 터를 식별 하기 위한 다음 세대 시퀀싱 기반 프로토콜 및 hygromycin 저항 유전자는 메 틸 CpG 바인딩 단백질 2 (융합 MeCP2) 비활동 성 X 염색체에 유전자입니다.