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DOI :
10.3791/56409-v
•
8:47 min
December 7th, 2017
Chapters
0:05
Title
1:00
Primary Neuron Culture and Brain Tissue Isolation
2:56
Tissue Digestion, Plating, and Culture
4:32
Live Image Recording
6:48
Results: The Snapshots of GFP-GLUT4 in Hypothalamic Neurons
7:47
Conclusion
このプロトコルは、タグ付きの観測リアルタイム緑色蛍光タンパク質 (GFP) のブドウ糖の運送者 4 (GLUT4) タンパク質インスリン刺激とインスリン-GLUT4 の CCR5 の生物学的役割の評価時に人身売買の手法を記述します。デコンボリューション顕微鏡を用いたシグナル伝達。
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