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DOI :
10.3791/56421-v
January 31st, 2018
Chapters
0:05
Title
1:00
Monomeric Avidin Fusion Protein Expression, Purification, and Refolding
3:53
Biotinylation of BCG Cell Surface
5:47
Binding of Monomeric Adivin-fusion Protein to Biotinylated BCG Surface
7:06
Results: Biotinylation with Avidin-OVA
9:13
Conclusion
迅速抗原細菌表面に表示のための新たな手法を提示することで、単量体のアビジンとの融合に興味の蛋白質への暴露に続いて表面ビオチン化が含まれます。選択した抗原と BCG を正常に読み込み表面加飾が従来の遺伝的アプローチを置き換えることができますを示唆して、その免疫原性が向上します。
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