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DOI :
10.3791/56464-v
November 26th, 2017
Chapters
0:05
Title
0:53
Liquid Culture for Treatment with RNAi Starting at L1
2:40
Collection of Young and Aged Worms Subjected to RNAi in Liquid Culture
6:14
Disruption of Animals
6:58
Isolation of SDS-insoluble Proteins for Mass Spectrometry
10:03
Results: Reduced IIS Prevents Age-dependent Protein Aggregation
11:26
Conclusion
本文所提出的方法的目的是在模型生物体的正常衰老过程中, 探索蛋白质的聚集性, 即线虫。该协议代表了一个强大的工具, 以研究高度不溶性的大骨料, 形成与年龄和确定如何改变 proteostasis 影响蛋白质聚集。
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