Name: real-time breath analysis by using secondary nanoelectrospray ionization coupled to high resolution mass spectrometry
Uploaded: 2018-03-09T12:30:00
Description: Watch this Scientific Journal Video about Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry at JoVE.com

Bu eser mali Ulusal Doğa Bilimleri Vakfı Çin tarafından (No. 91543117) desteklemiştir.

Dikkat: Lütfen ilgili tüm malzeme güvenlik bilgi formları (MSDS) kullanmadan önce danışın. Lütfen uygun kişisel koruyucu ekipman, örneğin, önlük, eldiven, gözlük, tam uzunlukta pantolon ve kapalı-toe ayakkabı kullanın).
1. sn-nanoESI kaynağını ayarlayın
<ol><li>Sn-nanoESI kaynak Ayrılmıştır süreci, Yani, göre ayarlayın nefes gaz bir electrospray tüy kesiştiği tanıştırdı ve şarj edilmiş damlacıkları (şekil 1</str...

<ol>
	<li>De Lacy Costello, B., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+review+of+the+volatiles+from+the+healthy+human+body.">A review of the volatiles from the healthy human body.</a> J. Breath Res. 8 (1), 014001-014030 (2014).</li><li>Phillips, M., Greenberg, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ion-trap+detection+of+volatile+organic+compounds+in+alveolar+breath.">Ion-trap detection of volatile organic compounds in alveolar breath.</a> Clin. Chem. 38 (1), 60-65 (1992).</li><li>Mart&#237;nez-Lozano Sinues, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Circadian+variation+of+the+human+metabolome+captured+by+real-time+breath+analysis.">Circadian variation of the human metabolome captured by real-time breath analysis.</a> PLoS One. 9 (12), 0114422-0114438 (2014).</li><li>Garcia-Gomez, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Secondary+electrospray+ionization+coupled+to+high-resolution+mass+spectrometry+reveals+tryptophan+pathway+metabolites+in+exhaled+human+breath.">Secondary electrospray ionization coupled to high-resolution mass spectrometry reveals tryptophan pathway metabolites in exhaled human breath.</a> Chem. Common. 52 (55), 8526-8528 (2016).</li><li>Garcia-Gomez, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-time+quantification+of+amino+acids+in+the+exhalome+by+secondary+electrospray+ionization-mass+spectrometry:+A+proof-of-principle+Study.">Real-time quantification of amino acids in the exhalome by secondary electrospray ionization-mass spectrometry: A proof-of-principle Study.</a> Clin. Chem. 62 (9), 1230-1237 (2016).</li><li>Mart&#237;nez-Lozano Sinues, P., Kohler, M., Brown, S. A., Zenobia, R., Dallmann, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gauging+circadian+variation+in+ketamine+metabolism+by+real-time+breath+analysis.">Gauging circadian variation in ketamine metabolism by real-time breath analysis.</a> Chem. Common. 53 (14), 2264-2267 (2017).</li><li>Gamez, G., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-time,+in+vivo+monitoring+and+pharmacokinetics+of+valproic+acid+via+a+novel+biomarker+in+exhaled+breath.">Real-time, in vivo monitoring and pharmacokinetics of valproic acid via a novel biomarker in exhaled breath.</a> Chem. Common. 47 (17), 4884-4886 (2011).</li><li>Li, X., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Drug+pharmacokinetics+determined+by+real-time+analysis+of+mouse+breath.">Drug pharmacokinetics determined by real-time analysis of mouse breath.</a> Angew. Chem. Int. Ed. 54 (27), 7815-7818 (2015).</li><li>Amorim, L. L. A., Cardeal, Z. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Breath+air+analysis+and+its+use+as+a+biomarker+in+biological+monitoring+of+occupational+and+environmental+exposure+to+chemical+agents.">Breath air analysis and its use as a biomarker in biological monitoring of occupational and environmental exposure to chemical agents.</a> J. Chromatogr. B. 853 (1-2), 1-9 (2007).</li><li>Bajtarevic, A., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+detection+of+lung+cancer+by+analysis+of+exhaled+breath.">Noninvasive detection of lung cancer by analysis of exhaled breath.</a> BMC Cancer. 9, 348 (2009).</li><li>Smith, D., Wang, T. S., Pysanenko, A., &#352;pan&#283;l, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+selected+ion+flow+tube+mass+spectrometry+study+of+ammonia+in+mouth-+and+nose-exhaled+breath+and+in+the+oral+cavity.">A selected ion flow tube mass spectrometry study of ammonia in mouth- and nose-exhaled breath and in the oral cavity.</a> Rapid Commun. Mass Spectrom. 22 (6), 783-789 (2008).</li><li>Li, X., Huang, L., Zhu, H., Zhou, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Direct+human+breath+analysis+by+secondary+nano-electrospray+ionization+ultrahigh+resolution+mass+spectrometry:+Importance+of+high+mass+resolution+and+mass+accuracy.">Direct human breath analysis by secondary nano-electrospray ionization ultrahigh resolution mass spectrometry: Importance of high mass resolution and mass accuracy.</a> Rapid Commun. Mass Spectrom. 31 (3), 301-308 (2017).</li><li>Mart&#237;nez-Lozano, P., Fernandez de la Mora, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrospray+ionization+of+volatiles+in+breath.">Electrospray ionization of volatiles in breath.</a> Int. J. Mass Spectrom. 265 (1), 68-72 (2007).</li><li>Zeng, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Detection+of+creatinine+in+exhaled+breath+of+humans+with+chronic+kidney+disease+by+extractive+electrospray+ionization+mass+spectrometry.">Detection of creatinine in exhaled breath of humans with chronic kidney disease by extractive electrospray ionization mass spectrometry.</a> J. Breath Res. 10 (1), 016008-016015 (2016).</li><li>Chen, H. W., Wortmann, A., Zhang, W. H., Zenobi, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapid+in+vivo+fingerprinting+of+nonvolatile+compounds+in+breath+by+extractive+electrospray+ionization+quadrupole+time-of-flight+mass+spectrometry.">Rapid in vivo fingerprinting of nonvolatile compounds in breath by extractive electrospray ionization quadrupole time-of-flight mass spectrometry.</a> Angew. Chem. Int. Ed. 46 (4), 580-583 (2007).</li><li>Benoi, F. M., Davldson, W. R., Lovett, A. M., Nacson, S., Ngo, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Breath+analysis+by+atmospheric+pressure+ionization+mass+spectrometry.">Breath analysis by atmospheric pressure ionization mass spectrometry.</a> Anal. Chem. 55 (4), 805-807 (1983).</li><li>Bregy, L., Mart&#237;nez-Lozano Sinues, P., Nudnova, M. M., Zenobi, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Real-time+breath+analysis+with+active+capillary+plasma+ionization-ambient+mass+spectrometry.">Real-time breath analysis with active capillary plasma ionization-ambient mass spectrometry.</a> J. Breath Res. 8 (2), 027102-027110 (2014).</li><li>Gaugg, M. T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Expanding+metabolite+coverage+of+real-time+breath+analysis+by+coupling+a+universal+secondary+electrospray+ionization+source+and+high+resolution+mass+spectrometry-a+pilot+study+on+tobacco+smokers.">Expanding metabolite coverage of real-time breath analysis by coupling a universal secondary electrospray ionization source and high resolution mass spectrometry-a pilot study on tobacco smokers.</a> J. Breath Res. 10 (1), 016010-016020 (2016).</li><li>Mart&#237;nez-Lozano, P., Zingaro, L., Finiguerra, A., Cristoni, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Secondary+electrospray+ionization-mass+spectrometry:+breath+study+on+a+control+group.">Secondary electrospray ionization-mass spectrometry: breath study on a control group.</a> J. Breath Res. 5 (1), 016002-016012 (2011).</li><li>Mart&#237;nez-Lozano Sinues, P., Zenobi, R., Kohler, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Analysis+of+the+exhalome+a+diagnostic+tool+of+the+future.">Analysis of the exhalome a diagnostic tool of the future.</a> Chest. 144 (3), 746-749 (2013).</li><li>Mart&#237;nez-Lozano Sinues, P., Fernandez de la Mora, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Direct+analysis+of+fatty+acid+vapors+in+breath+by+electrospray+ionization+and+atmospheric+pressure+Ionization-Mass+Spectrometry.">Direct analysis of fatty acid vapors in breath by electrospray ionization and atmospheric pressure Ionization-Mass Spectrometry.</a> Anal. Chem. 80 (21), 8210-8215 (2008).</li><li>Mart&#237;nez-Lozano Sinues, P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Breath+analysis+in+real+time+by+mass+spectrometry+in+chronic+obstructive+pulmonary+disease.">Breath analysis in real time by mass spectrometry in chronic obstructive pulmonary disease.</a> Respiration. 87 (4), 301-310 (2014).</li><li>Mart&#237;nez-Lozano Sinues, P., Kohler, M., Zenobi, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monitoring+diurnal+changes+in+exhaled+human+breath.">Monitoring diurnal changes in exhaled human breath.</a> Anal. Chem. 85 (1), 369-373 (2013).</li><li>Chen, H. W., Zenobi, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Neutral+desorption+sampling+of+biological+surfaces+for+rapid+chemical+characterization+by+extractive+electropray+ionization+mass+spectrometry.">Neutral desorption sampling of biological surfaces for rapid chemical characterization by extractive electropray ionization mass spectrometry.</a> Nat. Protoc. 3 (9), 1467-1475 (2008).</li><li>Li, X., Hu, B., Ding, J., Chen, H. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rapid+characterization+of+complex+viscous+samples+at+molecular+levels+by+neutral+desorption+extractive+electrospray+ionization+mass+spectrometry.">Rapid characterization of complex viscous samples at molecular levels by neutral desorption extractive electrospray ionization mass spectrometry.</a> Nat. Protoc. 7 (6), 1010-1025 (2011).</li><li>Gordon, S. M., Szidon, J. P., Krotoszynski, B. K., Gibbons, R. D., O'Neill, H. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Volatile+organic+compounds+in+exhaled+air+from+patients+with+lung+cancer.">Volatile organic compounds in exhaled air from patients with lung cancer.</a> Clin. Chem. 31 (8), 1278-1282 (1985).</li><li>Ding, J. H., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+extractive+electrospray+ionization+ion+trap+mass+spectrometry+in+vivo+breath+analysis.">Development of extractive electrospray ionization ion trap mass spectrometry in vivo breath analysis.</a> Analyst. 134 (10), 2040-2050 (2009).</li><li>Basum, G., Dahnke, H., Halmer, D., Hering, P., M&#252;rtz, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Online+recording+of+ethane+trances+in+human+breath+via+infrared+laser+spectroscopy.">Online recording of ethane trances in human breath via infrared laser spectroscopy.</a> J. Appl. Physiol. 95 (6), 2583-2590 (2003).</li><li>T&#248;ien, &. #. 2. 1. 6. ;. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Automated+open+flow+respirometry+in+continuous+and+long-term+measurements:+design+and+principles.">Automated open flow respirometry in continuous and long-term measurements: design and principles.</a> J. Appl. Physiol. 114 (8), 1094-1107 (2013).</li><li>Huang, L., Li, X., Xu, M., Huang, Z. X., Zhou, Z. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identification+of+relatively+high+molecular+weight+compounds+in+human+breath+using+secondary+nano+electrospray+ionization+ultrahigh+resolution+mass+spectrometry.">Identification of relatively high molecular weight compounds in human breath using secondary nano electrospray ionization ultrahigh resolution mass spectrometry.</a> Chem. J. Chinese U. 38 (5), 752-757 (2017).</li></ol>

Bir ticari nanoESI kaynağını temel alan sn-nanoESI kaynak oluşturmak yoluyla, iyonlaşma verimliliği bir ESI kaynak30kullanarak daha yüksek olduğunu. İşlem yalıtarak gibi Ayrıca, iyonlaşma verimliliği daha da kapalı bir odaya artırıldı ortam arka plan havadan ve aynı zaman gaz örnek ve sprey tüy arasında karıştırma kolaylaştırır. Sn nanoESI kullanarak, daha az parametrelerini optimize yükleme, uygulama ve bakım için daha kolay bir ESI kaynağına göre gerekir.
<p...

Modern analitik teknikler hızlı gelişimi ile uçucu organik bileşikler (VOC) yüzlerce insan dışarı solunan nefes1' de tespit edilmiştir. Bu UOB'ler çoğunlukla alveoler hava (sağlıklı bir yetişkin için ~ 350 mL) kaynaklanan ve anatomik ölü boşluk hava (~ 150 mL)2, hangi vücut metabolizma3,4,5,6tarafından,7 etkilenir ,8 ve çevre kirliliği9, anılan sıraya göre. Sonuç olarak, tespit, bu UOB'ler biyolojik hastalık tanı ve çevre pozlama için non-invaziv bir şekilde kullanılmak üzere umut vericidir.
Gaz Kromatografi Kütle spektrometresi (GC-MS) dışarı solunan UOB'ler2, gerçek zamanlı nefes analiz için geliştirilmiştir, doğrudan MS teknikleri, nitel ve nicel analiz için en çok kullanılan teknik olsa avantajları var zaman yüksek çözünürlük ve basit örnek ön hazırlık. Proton transferi tepki MS (PTR-MS)10, gibi doğrudan MS teknikleri iyon akışı tüp MS (SIFT-MS)11, ikincil electrospray iyonlaşma MS (SESI-MS)12,13 (Ekstraktif electrospray da adlandırılan seçili İyonlaşma MS, EESI-MS14,15), izleme atmosferik gaz analizörü (TAG'dan)16 ve plazma iyonizasyon MS (PI-MS)17 araştırdık son yıllarda.
Tüm doğrudan MS teknikleri arasında SESI bir evrensel yumuşak iyonlaşma tekniği19,20,21bilinen; ve kaynak özelleştirilebilir ve Kütle Spektrometreler, örneğin, zaman uçuş Kütle Spektrometre8,15farklı türde birleştiğinde kolaydır, iyon kapanı Kütle Spektrometre14 ve orbitrap Kütle Spektrometre12 ,18. Şu ana kadar SESI-MS başarıyla solunum yolu hastalıkları22, tanılamada sirkadiyen ritim3,6,23, farmakokinetik7,8, ölçme kullanılmıştır ve açığa metabolik yolu4, vb. Son olarak, bir ticari SESI kaynağı kullanılabilir hale gelmiştir.
Bu çalışmada, bir ikincil nanoelectrospray facile ve kompakt iyonizasyon kaynağı (sn-nanoESI) kurmak ve yüksek çözünürlüklü bir Kütle Spektrometre birleştiğinde. Dışarı solunan UOB'ler nefeste gerçek zamanlı ölçümleri sunuldu.

<table>
	<tbody>
		<tr>
			<td>Ultrapure water</td>
			<td>Merck Millipore, USA</td>
			<td>MPGP04001</td>
			<td>Resistance &#62;18.2 M&#937;&#183;cm</td>
		</tr>
		<tr>
			<td>Formic acid</td>
			<td>Sigma-Aldrich, USA</td>
			<td>F0507</td>
			<td>Corrosive to the respiratory tract.</td>
		</tr>
		<tr>
			<td>Nitrogen gas</td>
			<td>Guangzhou Shiyuan Gas Co. Ltd., China</td>
			<td>N.A.a</td>
			<td>Purity &#62;99.99%</td>
		</tr>
		<tr>
			<td>Q Exactive hybrid quadrupole-orbitrap mass spectrometer</td>
			<td>Thermo Scientific, USA</td>
			<td>02634L(S/N)</td>
			<td>Beware of high voltage and high temperature</td>
		</tr>
		<tr>
			<td>NanoESI source</td>
			<td>Thermo Scientific, USA</td>
			<td>ES002373(S/N); ES071(P/N)</td>
			<td>Beware of high voltage and high temperature</td>
		</tr>
		<tr>
			<td>Nano LC pump</td>
			<td>Thermo Scientific, USA</td>
			<td>5041.0010A(P/N)</td>
			<td>/</td>
		</tr>
		<tr>
			<td>Xcalibur software (Version 3.0)</td>
			<td>Thermo Scientific, USA</td>
			<td>BRE0008596</td>
			<td>/</td>
		</tr>
		<tr>
			<td>Dino-Lite Digital Microscope</td>
			<td>Tech Video System (SuZhou) Co.Ltd., China</td>
			<td>CQ401833R(S/N)</td>
			<td>/</td>
		</tr>
		<tr>
			<td>Nafion tubing</td>
			<td>Perma Pure LLC, USA</td>
			<td>ME60</td>
			<td>/</td>
		</tr>
		<tr>
			<td>PTFE tubing (I.D. 4 mm)</td>
			<td>Dongguan Hongfu Insulating Material Co. Ltd., China</td>
			<td>N.A.</td>
			<td>Beware of the possible loss of polar compounds</td>
		</tr>
		<tr>
			<td>Mass flow controller</td>
			<td>Line-Tech, Korea</td>
			<td>M15122007 (S/N)</td>
			<td>/</td>
		</tr>
		<tr>
			<td>Flow meter</td>
			<td>Yuyao Industrial Automation Meter Factory, China</td>
			<td>40784</td>
			<td>/</td>
		</tr>
		<tr>
			<td>aN.A.: not available.</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

real-time breath analysis by using secondary nanoelectrospray ionization coupled to high resolution mass spectrometry

Onlar non-invaziv bir şekilde hastalık tanı ve çevre pozlama için biyolojik olarak hizmet verebilir beri dışarı solunan uçucu organik bileşikler (VOC) büyük ilgi uyandırdı. Bu çalışmada, biz ikincil nanoelectrospray iyonlaşma yüksek çözünürlüklü kütle spektrometresi (sn-nanoESI-HRMS) birleştiğinde kullanarak gerçek zamanlı dışarı solunan UOB'ler karakterize etmek için bir iletişim kuralı mevcut. Ev yapımı sn-nanoESI kaynak kolayca bir ticari nanoESI kaynağına göre kurulmuştur. Tepeler yüzlerce arka plan düşülen kitle spectra dışarı solunan nefes içinde gözlendi ve kitle doğruluk değerlerdir -4.0-13.5 ppm ve-20.3-pozitif ve negatif iyon algılama modlarında 1.3 ppm anılan sıraya göre. Doruklarına doğru kitle ve izotopik desen göre doğru elemental bileşimi ile ayrıldı. 30'dan az s bir soluk verme ölçüm için kullanılan ve altı çoğaltılmış ölçümler için yaklaşık 7 dakika sürer.

Şekil 3 m/z 50-750 her iki pozitif ve negatif iyon algılama modu altında kaydedilen kitle aralığında nefes parmak gösterir. 291 tepeler (en yüksek yoğunluk > 5.0x104) ve 173 tepeler (en yüksek yoğunluk > 3.0x104) arka plan düşülen nefes parmak pozitif ve negatif iyon algılama modlarında içinde sırasıyla gözlenmiştir. Kitle spectra Peaks'e tanımlamak için önceden yayınlar için Ayrıntılar<sup class="xre...

Watch this Scientific Journal Video about Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry at JoVE.com

Real-time Breath Analysis by Using Secondary Nanoelectrospray Ionization Coupled to High Resolution Mass Spectrometry

İkincil nanoelectrospray iyonlaşma kullanarak gerçek zamanlı dışarı solunan nefes kimyasal bileşimi karakterize bir protokol kütle spektrometresi gösterdi yüksek çözünürlüklü birleştiğinde.

İkincil Nanoelectrospray iyonlaşma kullanarak gerçek zamanlı nefes analiz için yüksek çözünürlüklü kütle spektrometresi birleştiğinde

Exhaled volatile organic compounds (VOCs) have aroused considerable interest, since they can serve as biomarkers for disease diagnosis and environmental ...

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Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Ultra Performanslı Sıvı Kromatografi-Yüksek Çözünürlüklü Kütle Spektrometre (UPLC-HRMS) kullanarak Biyolojik Kaynaklar gelen hedefsiz Metabolomik

Here we present a workflow to analyze the metabolic profiles for biological samples of interest including; cells, serum, or tissue. The sample is first ...

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In this study we evaluated a method for the characterization of complexes, formed in different relative ratios of mercury(II) to dicysteinyl tetrapeptide, ...

Rapid High-throughput Species Identification of Botanical Material Using Direct Analysis in Real Time High Resolution Mass Spectrometry

Gerçek Zamanlı Yüksek Çözünürlüklü Kütle Spektrometresi Doğrudan Analizi Kullanılarak Botanik Malzeme Hızlı Yüksek verim Türler Kimlik

We demonstrate that direct analysis in real time-high resolution mass spectrometry can be used to produce mass spectral profiles of botanical material, ...

Fizzy Extraction of Volatile Organic Compounds Combined with Atmospheric Pressure Chemical Ionization Quadrupole Mass Spectrometry

Atmosferik Basınçlı Kimyasal İyonlaşma Kuvveti Kütle Spektrometresi ile Birleştirilen Uçucu Organik Bileşiklerin Özsu Çıkarma

Chemical analysis of volatile and semivolatile compounds dissolved in liquid samples can be challenging. The dissolved components need to be brought to ...

Synthesis and Mass Spectrometry Analysis of Oligo-peptoids

Sentez ve kütle spektrometresi analizleri Oligo-peptoids

Peptoids are sequence-controlled peptide-mimicking oligomers consisting of N-alkylated glycine units. Among many potential applications, peptoids have ...

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) Mass Spectrometry

Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight MALDI-TOF Mass Spectrometry

Matris ile sentetik polimerler karakterizasyonu Lazer Desorpsiyon iyonlaşma zaman uçuş (MALDI-TOF) kütle spektrometresi destekli

There are many techniques that can be employed in the characterization of synthetic homopolymers, but few provide as useful of information for end group ...

An Efficient Sample Preparation Method to Enhance Carbohydrate Ion Signals in Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

Matris yardımlı Lazer Desorpsiyon/iyonlaşma kütle spektrometresi karbonhidrat iyon sinyalleri geliştirmek için bir verimli örnek hazırlama yöntemi

Sample preparation is a critical process in mass spectrometry (MS) analysis of carbohydrates. Although matrix-assisted laser desorption/ionization (MALDI) ...

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Multisegment enjeksiyon-kılcal Elektroforez kütle spektrometresi kullanılarak yüksek üretilen iş ve kapsamlı uyuşturucu gözetim

New analytical methods are urgently needed to enable high-throughput, yet comprehensive drug screening, given an alarming opioid and prescription drug ...