Name: regenerative therapy by suprachoroidal cell autograft in dry age-related macular degeneration: preliminary in vivo report
Uploaded: 2018-02-12T12:30:00
Description: Watch this Scientific Journal Video about Regenerative Therapy by Suprachoroidal Cell Autograft in Dry Age-related Macular Degeneration: Preliminary In Vivo Report at JoVE.com

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Undersøgelse-protokollen blev godkendt af den etiske komité i lav Vision Academy og alle fag underskrevet et skriftligt samtykke i overensstemmelse med Helsinki-erklæringen. Denne undersøgelse har fået etiske godkendelse fra både Loughborough og Sheffield universiteter.
Bemærk: De medtagelse og udelukkelse kriterier af tørre aldersrelateret makuladegeneration patienter til at modtage suprachoroidal autologt transplantat af Limoli Retinal restaurering teknik (LRRT) er beskrevet i <stron...

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Det primære formål med denne undersøgelse var at vurdere, om suprachoroidal graft af adipocytter, ADSCs i SVF og PRP kunne forbedre VA og retinal følsomhed i tørre AMD-ramte øjne over tid. Et andet hovedformål var at vise mulige terapeutiske virkninger af disse celler, baseret på den seneste litteratur, da flere prækliniske undersøgelser har antydet, at GF-baseret terapi kan være nyttigt for patientpleje i flere sygdomme.
I virkeligheden, nogle undersøgelser har vist, at autologe h...

Celleterapi, bestående af systemiske eller lokale injektion af stem/stamceller i det skadede område at behandle flere kroniske lidelser, har henledt opmærksomhed i de sidste ti år1. Siden 1990 ' erne, er vækstfaktorer (GFs) blevet undersøgt for deres potentielt terapeutisk rolle i retinal atrofi2. I virkeligheden, kan mange menneskelige celler producere GFs, som er specifikke proteiner, der er i stand til at blokere eller sinke apoptose, dvs., de programmerede død af celler3.
Det er kendt, at tørre aldersrelateret Macula degeneration (AMD) er en atrofisk retinal sygdom hvor gradvis og uigenkaldeligt celledød involverer skade fotoreceptor laget og følgelig tab af centrale visuelle funktion4. AMD er den hyppigste årsag til blindhed i mennesker over 55 år i udviklede lande og tegner sig for 80% af alle Macula degenerations, som mangler en effektiv behandling til dato.
Flere undersøgelser har vist, at der er forskellige kilder, hvor der kan indhentes autolog GFs. Disse omfatter forskellige celletyper, herunder fedt stromale celler udvundet af orbital fedt, trombocytter fremstillet af trombocyt-rich plasma (PRP) og fedt-afledte stamceller (ADSCs) indgår i stromale vaskulære fraktion (SVF) af fedtvæv5 ,6,7. Det aktuelle GF sæt sikrer retinale neuroenhancement og forskning udført af Filatov, Meduri, Pelaez, og Limoli har vist, at autologt fedt transplantation (AFT) er effektiv8,9,10.
Desuden en forudgående undersøgelse viste signifikante forbedringer i electroretinogram (ERG) data, registreres indlæg suprachoroidal autologt transplantat, i tørre AMD-ramte øjne11. Den kirurgisk podede væv i suprachoroidal rum moduleret paracrine sekretion af retinale celler, at forskyde deres apoptose6,7,12. Overvejer ydre nukleare lagtykkelse, den histologiske undersøgelse af nethinden i marsvin har vist at GFs kunne have en trofiske virkning på nethinden. Derfor, den direkte eller indirekte brug af GFs kan potentielt bringe terapeutiske fordele gennem et afbalanceret forhold mellem molekylære induktorer og hæmmere6,7,12.
Formålet med denne metode er at vurdere, om suprachoroidal graft af adipocytter, ADSCs i SVF og PRP kan forbedre bedst korrigerede synsstyrke (BCVA) og microperimetry (MY) svar i tørre AMD-ramte øjne. Denne undersøgelse har til formål at demonstrere det terapeutiske effekt af autograft på grundlag af dens GF produktion i henhold til den citerede litteratur6,7,12,13.

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			<td height="42" style="height:42px;width:216px;">Blunt cannula, 3 mm.&#160;</td>
			<td style="width:179px;">Mentor, Santa Barbara, CA.</td>
			<td style="width:119px;"></td>
			<td style="width:167px;"></td>
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		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Luer-LokTM syringe.&#160;</td>
			<td style="width:179px;">BD Biosciences, Franklin Lakes, NJ.</td>
			<td style="width:119px;"></td>
			<td></td>
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			<td height="42" style="height:42px;width:216px;">Regen-BCT tube.&#160;</td>
			<td style="width:179px;">RegenKit; RegenLab, Le Mont-sur-Lausanne, CH.</td>
			<td style="width:119px;"></td>
			<td></td>
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			<td height="63" style="height:63px;width:216px;">Centrifuge</td>
			<td style="width:179px;">&#160;RegenPRP Centri. RegenLab, Le Mont-sur-Lausanne, CH.</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">BD Venflon Pro Safety 22G x 1.00 inch (0.9 mm x 25 mm).&#160;</td>
			<td style="width:179px;">BD Biosciences, Franklin Lakes, NJ.</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">SPSS Statistics Version 19.0</td>
			<td style="width:179px;">IBM Corp., Armonk, NY, USA.</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Confocal scanning laser ophthalmoscope&#160;</td>
			<td style="width:179px;">Nidek Inc, Fremont, CA</td>
			<td style="width:119px;">Nidek F10&#160;</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Cirrus 5000 Spectral Domain-Optical Coherence Tomography</td>
			<td style="width:179px;">Carl Zeiss Meditec AG, Jena, Germany</td>
			<td style="width:119px;">&#160;SD-OCT&#160;</td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Maia 100809 Microperimetry&#160;</td>
			<td style="width:179px;">CenterVue S.p.A., Padua, Italy</td>
			<td style="width:119px;"></td>
			<td></td>
		</tr>
		<tr height="42">
			<td height="42" style="height:42px;width:216px;">Ocular electrophysiology electromedical system,</td>
			<td style="width:179px;">C.S.O., S.r.l., Scandicci, Italy</td>
			<td style="width:119px;"></td>
			<td>&#160;Retimax for ERG&#160;</td>
		</tr>
	</tbody>
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regenerative therapy by suprachoroidal cell autograft in dry age-related macular degeneration: preliminary in vivo report

Denne undersøgelse har til formål at undersøge om en suprachoroidal graft af autologe celler kan forbedre bedst korrigerede synsstyrke (BCVA) og svar til microperimetry (MY) i øjne påvirket af tørre aldersrelateret Macula Degeneration (AMD) over tid gennem den produktion og sekretion af vækstfaktorer (GFs) på omkringliggende væv. Patienter blev randomiseret til hver gruppe. Alle patienter blev diagnosticeret med tør AMD og BCVA lig med eller større end 1 logaritme af den mindste vinkel af opløsning (logMAR). En suprachoroidal autologt transplantat af Limoli Retinal restaurering teknik (LRRT) blev udført på gruppe A, som omfattede 11 øjne fra 11 patienter. Teknikken blev udført af implanterer adipocytter, fedtholdigt-afledte stamceller udvundet stromale vaskulære fraktion, og blodplader fra trombocyt-rich plasma i suprachoroidal rum. Omvendt, gruppe B, herunder 14 øjne af 14 patienter, blev brugt som en kontrolgruppe. For hver patient, blev diagnosen bekræftet af Konfokal scanning laser oftalmoskop og spektral domæne-optisk kohærens tomografi (SD-OCT). I gruppe A, BCVA forbedret af 0.581 til 0.504 på 90 dage, og 0.376 logMAR på 180 dage (+32.20%) efter operationen. Desuden, min test steget med 11.44 dB til 12.59 dB på 180 dage. De forskellige celletyper indpodet bag choroidea var i stand til at sikre konstant GF sekretion i choroidal flow. Derfor resultaterne tyder på, at visuel skarphed (VA) i gruppen podede kan stige mere end i kontrolgruppen efter seks måneder.

Ved hjælp af den procedure, der præsenteres her, blev to grupper af tør AMD-ramte patienter med BCVA lig med eller større end 1 logaritme af den mindste vinkel af opløsning (logMAR), indskrevet i undersøgelsen. Gruppe A, herunder 11 øjne af 11 patienter, modtagne suprachoroidal autologt transplantat af Limoli Retinal restaurering teknik (LRRT), mens gruppe B, herunder 14 øjne af 14 patienter, blev brugt som en kontrolgruppe.
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Watch this Scientific Journal Video about Regenerative Therapy by Suprachoroidal Cell Autograft in Dry Age-related Macular Degeneration: Preliminary In Vivo Report at JoVE.com

Regenerative Therapy by Suprachoroidal Cell Autograft in Dry Age-related Macular Degeneration: Preliminary In Vivo Report

Regenerativ terapi af Suprachoroidal celle Autograft i tørre aldersrelateret makuladegeneration: foreløbige In Vivo rapport

Målet med denne undersøgelse er at vurdere, om suprachoroidal graft fedtholdigt-afledte stamceller inkluderet i stromale vaskulære fraktion og trombocytter fremstillet af trombocyt-rich plasma ved Limoli Retinal restaurering teknik kan forbedre synsstyrke og retinal følsomhed reaktioner i øjnene påvirket af tørre aldersrelateret makuladegeneration.

This study is aimed at examining whether a suprachoroidal graft of autologous cells can improve best corrected visual acuity (BCVA) and responses to ...

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Regenerative Therapy by Suprachoroidal Cell Autograft in Dry Age-related Macular Degeneration: Preliminary In Vivo Report

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Sekventiel<em&gt; In vivo</em&gt; Imaging af Osteogene Stem / stamceller Under frakturreparation

Bone turns over continuously and is highly regenerative following injury. Osteogenic stem/progenitor cells have long been hypothesized to exist, but in ...

The Monoiodoacetate Model of Osteoarthritis Pain in the Mouse

Den Monoiodoacetate Model of Slidgigt Smerter i Mus

A major symptom of patients with osteoarthritis (OA) is pain that&#160;is triggered by peripheral as well as central changes within the pain pathways. The ...

Depletion of Mouse Cells from Human Tumor Xenografts Significantly Improves Downstream Analysis of Target Cells

Udtømning af Mouse Celler fra Humane tumorxenoplantater Markant Forbedrer Downstream Analyse af Target Cells

The use of in vitro cell line models for cancer research has been a useful tool. However, it has been shown that these models fail to reliably mimic ...

A Method for Quantifying Upper Limb Performance in Daily Life Using Accelerometers

En metode til kvantificering Upper Limb Performance i min hverdag anvender accelerometre

A key reason for referral to rehabilitation services after stroke and other neurological conditions is to improve one's ability to function in daily life. ...