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DOI :
10.3791/56471-v
•
7:20 min
February 4th, 2018
Chapters
0:00
Title
1:42
DNA Isolation from Ticks
4:06
Nested PCR Detection of Borrelia OspA and FlaB
5:25
Results
6:22
Conclusions
巢式 PCR 是一种灵敏的、特异的、直接的技术, 可以应用于蜱 DNA 提取物来探测莱姆病的致病剂螺旋体螺旋体。最初的 PCR 实验使用基因特异的引物产生长的增, 然后成为模板为随后反应使用内部引物。
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