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DOI :
10.3791/56474-v
November 15th, 2017
Chapters
0:05
Title
1:00
Preparation of Cells/Tissues
1:45
Protein Extraction, Quality Control Western Blotting, In-solution Digestion, and Peptide Desalting
4:45
TMT Labeling of Peptides
5:50
Extensive High-resolution, Basic pH LC Prefactionation
6:43
LC-MS/MS Preparation and Parameters
7:41
Results: Deep Proteome Profiling Analysis
8:45
Conclusion
我们提出了一个协议, 以准确定量蛋白质与等压标签, 广泛的分馏, 生物信息学工具, 和质量控制步骤, 结合液相色谱与高分辨率质谱仪接口。
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