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DOI :
10.3791/56484-v
September 27th, 2017
Chapters
0:05
Title
0:56
Collection of Placental Tissue
1:42
Isolation of Trophoblasts from Vilous Tissue
5:11
Density Centrifugation
6:29
Plating Cells
7:18
Treating Cells with TNFα and Harvesting Cells and Total Protein
9:10
Results: TNFα Regulates the Expression of Rubicon in Trophoblasts from Lean Pregnancies with Female Fetuses
10:20
Conclusion
ここで紹介は一次電池文化のよりよくの分離に続いてひと胎盤絨毛組織のサンプリングのためのプロトコルです。Tnf α と絨毛トロホブ ラストの治療肥満の子宮内環境で炎症を繰り返すし、母性的な肥満と胎盤の炎症によって調整される分子標的の探索が容易になります。
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