Name: gene regulation and targeted therapy in gastric cancer peritoneal metastasis: radiological findings from dual energy ct and pet/ct
Uploaded: 2018-01-22T14:00:00
Description: Watch this Scientific Journal Video about Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT at JoVE.com

这项工作得到了自然科学基金 (No。U1532107) 与上海交通大学生物医学工程项目 (No。YG2014MS53)。作者感谢叶剑英李和燕神对 DECT 和 PET/CT 成像方法的有益评价和技术支持。

这项工作是严格按照上海交通大学实验动物保护和使用指南所制定的标准进行的, 并获得瑞金医院实验室动物伦理委员会批准。
1. 胃癌腹膜转移动物模型
<ol><li>将一个适度分化的 SGC-7901 人胃癌细胞系分为 SGC-7901/sFRP1 组和 SGC-7901/vector 组。培养两组细胞分别在 RPMI 1640 补充10% 胎牛血清, 100 单位/毫升链霉素, 和100µg/毫升青霉素在37° c 的湿化气氛与 5% CO2。</li>
	<li>使用4-...

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动物模型在胃癌的分子机制研究中得到了广泛的应用, 并尝试了各种治疗策略23,24,25。在这项研究中, 我们描述了一个详细的协议, 胃癌腹膜转移裸鼠模型, 使用 DECT 和 PET/CT 图像胃肿瘤实时识别肿瘤细胞增殖, 并监测腹膜转移和胃癌动物模型的治疗干预反应。这种方法可以使参与研究胃癌的分子机制的研究人员, 或实验成像肿瘤, ...

胃癌 (GC) 仍然是第四最常见的恶性肿瘤, 也是全球癌症死亡率的第二大原因1。虽然胃癌的诊断和治疗的准确性有了很大的提高, 但腹膜转移是胃癌预后和复发的最关键因素, 是术后死亡的决定性指标2。人们普遍认为腹膜传播是一种危及生命的转移方式, 其中, 一旦建立腹膜播散, 疾病就无法控制, 病人的预后较差。因此, 胃癌腹膜转移的检测和疗效评价是临床应用的关键。
胃癌发病率和死亡率的上升促使研究人员识别其分子机制。高表达的基因, 如分泌的卷曲相关蛋白 1 (sFRP1) 可能导致激活的信号通路在早期阶段的胃癌, 促进肿瘤的生长, 增殖, 分化和凋亡的过程中3,4,5,6,7. sFRP1-overexpression 细胞显示 TGFβ的表达增加, 其下游目标, 和 TGFβ介导的急诊室8。以往的研究表明, TGF-β1水平与腹膜转移和胃癌 TNM 分期相关。我们描述了 sFRP1 过度表达和 TGF-β1抑制的癌细胞增殖的变化, 并建立了腹膜转移的动物模型, 以显示肿瘤在基因调控作用下的影像表现。
动物模型的胃癌是必不可少的工具, 研究肿瘤的发展和试验的各种治疗策略, 而不必牺牲动物。动物模型在研究肿瘤和起源细胞的形成机制、确定肿瘤干细胞的存在以及研究各种新的治疗策略方面证明是有用的。因此, 实时无创技术可以准确描述胃肿瘤的发展和治疗的肿瘤反应, 从而确定裸鼠腹膜转移结节的发展, 并监测肿瘤反应的各种实验和治疗干预。
目前, 多探测器 CT (MDCT) 在胃癌 TNM 分期中起着重要作用, 对预测肿瘤切除术后9有一定的参考价值。然而, 经病理证实的胃癌患者的影像学研究主要基于形态学。DECT 成像扩展参数, 以反映功能信息, 提供单色图像, 并可能有助于提高 N 分期准确性胃癌。此外, 这项技术将有助于获得材料分解图像, 这可能有助于区分分化和未分化的胃癌, 并之间转移和非转移淋巴结10.随着 DECT 的引入, CT 的功能成像方面也被添加到临床应用中, 有助于评价疗效和预测患者预后11,12,13。PET/CT 是一种有用的成像技术的检测和分期胃癌, 并能评估复发的肿瘤有效14。肿瘤细胞增殖和血管生成都被认为是必要的, 在开发一个可检测的肿瘤15, 肿瘤结节显示了一个积极的性能与更高的 SUVmax的 PET/CT. 基于对好氧的偏好糖酵解, 18F-葡萄糖的模拟, 已被利用作为一个有希望的示踪剂的诊断恶性肿瘤, 结合 PET/CT16。这种方法依赖于肿瘤组织的快速葡萄糖消耗, 并具有广泛的临床应用, 包括协助检测、分期和评估肿瘤的预后, 以及监测肿瘤对治疗的反应17,18. 由于非侵入性的方法, DECT 和 PET/CT 已被用于诊断恶性肿瘤和评估肿瘤对各种疗法的反应。
我们的小组一直使用这种非侵入性成像方法与 DECT 和 PET/CT 扫描仪, 以检测和监测的过程中的肿瘤生长和转移的活鼠19。我们探讨了 sFRP1-overexpression 诱导的胃癌细胞的影像学表现在体内使用裸鼠, 与 DECT 和 PET/CT, 并描述了 SUVmax值的变化后, TGF-β1抑制剂的靶向治疗, 以确认研究了基因诱导后腹膜肿瘤结节的发展, 并对实验性治疗中肿瘤结节的变化进行了探讨。本文对小鼠胃肿瘤腹膜转移的建模、DECT 和 PET/CT 的检测与监测提供了详细的方法。

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			<td height="21" style="height:21px;width:247px;">Iohexol</td>
			<td style="width:389px;">BEJING BEILU PHARMACEUTICAL CO,LTD</td>
			<td style="width:136px;">NMPN:H20053800</td>
			<td style="width:423px;">non-ionic contrast medium for DECT scan</td>
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			<td height="21" style="height:21px;width:247px;">normal saline</td>
			<td style="width:389px;">HUNAN KELUN PHARMACEUTICAL CO,LTD</td>
			<td style="width:136px;">NMPN:H43020455</td>
			<td>placebo of control group</td>
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			<td height="21" style="height:21px;width:247px;">BALB/c nude mice&#160;</td>
			<td style="width:389px;">SLAC LABORATORY ANIMAL</td>
			<td style="width:136px;">BALB/cASlac-nu</td>
			<td>animal model</td>
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			<td height="30" style="height:31px;width:247px;">SGC-7901&#160; cells</td>
			<td style="width:389px;">Library of typical culture of Chinese academy of sciences</td>
			<td style="width:136px;">TCHu 46</td>
			<td>gastric cancer cell&#160;</td>
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		<tr height="21">
			<td height="21" style="height:21px;width:247px;">SB431542</td>
			<td style="width:389px;">Selleck</td>
			<td style="width:136px;">No.S1067</td>
			<td>TGF-&#946;1 inhibitor</td>
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			<td height="21" style="height:21px;width:247px;">GE Discovery CT750 HD</td>
			<td style="width:389px;">GE Healthcare</td>
			<td style="width:136px;"></td>
			<td>dual-energy spectral CT scanner&#160;</td>
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			<td height="21" style="height:21px;width:247px;">AW Volumeshare5</td>
			<td style="width:389px;">GE Healthcare</td>
			<td style="width:136px;"></td>
			<td>dual-energy spectral CT workstation</td>
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			<td height="21" style="height:21px;width:247px;">Siemens Inveon micro-PET/CT</td>
			<td style="width:389px;">Siemens Preclinical Solution</td>
			<td style="width:136px;"></td>
			<td>positron emission tomography/ 
			computed tomography scanner&#160;</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:247px;">Inveon Acquisition Workplace</td>
			<td style="width:389px;">Siemens Preclinical Solution</td>
			<td style="width:136px;"></td>
			<td>PET-CT workstation</td>
		</tr>
	</tbody>
</table>

gene regulation and targeted therapy in gastric cancer peritoneal metastasis: radiological findings from dual energy ct and pet/ct

胃癌在全世界的癌症发病率中仍然是第四, 五年的存活率只有 20%-30%。腹膜转移是胃癌最常见的转移类型, 是预后的决定性因素。预防和控制腹膜转移的发展, 有助于延长胃癌患者的生存期。非侵入性和高效的影像学技术将帮助我们识别腹膜转移的侵袭和转移过程, 并监测肿瘤结节的变化, 以应对治疗。这将使我们能够准确地描述胃癌的发展过程和分子机制。我们最近用双能量 CT (DECT) 和正电子发射断层扫描/计算机断层扫描 (PET/ct) 平台进行了实验, 以检测和监测裸鼠胃肿瘤转移的模型。我们已经表明, 每周连续监测与 DECT 和 PET/CT 可以识别动态变化的腹膜转移。sFRP1-overexpression 在胃癌模型中的作用表现为阳性的放射性表现, 较高的葡萄糖摄取和增强, 而 SUV 的max (标准摄取值) 显示出明显的改变趋势对 TGF-β1抑制剂靶向治疗的反应。在本文中, 我们描述了详细的非侵入性成像程序, 以进行更复杂的研究胃癌腹膜转移使用动物模型, 并提供了代表性的成像结果。利用非侵入性成像技术, 我们应该能够更好地了解肿瘤发生的机制, 监测癌症的生长, 并评估治疗干预对胃癌的影响。

在两周的细胞线注射后, 对裸鼠进行了 DECT 和 PET/CT 扫描。影像学图像在 sFRP1 过度表达组的腹部轮廓外显示皮下转移, 并通过彩色比例图像 (图 1-c) 进行外周增强的转移, 取得了良好的效果。PET/CT 图像描述局部异常葡萄糖摄取转移, 包括在腹膜和皮下转移 (图 1d)。DECT 和 PET/CT 图像上显示的腹膜转移和大皮...

Watch this Scientific Journal Video about Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT at JoVE.com

Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT

本协议描述了双能量 ct 和 PET/ct 成像方法在肿瘤成像和疗效评价中的价值。本文阐述了双能量 ct 和 PET/ct 对胃癌腹膜转移的基因调控和靶向治疗的研究方法和结果。

胃癌腹膜转移的基因调控及靶向治疗: 双能量 ct 与 PET/ct 的影像学研究

Gastric cancer remains fourth in cancer incidence worldwide with a five-year survival of only 20%-30%. Peritoneal metastasis is the most frequent type of ...

Research

Education

JoVE Journal

JoVE Core

Cell Biology

Medicine

Unit 1

Cancer

SCIENTISTS IN ACTION

Establishment and Propagation of Human Retinoblastoma Tumors in Immune Deficient Mice

在免疫缺陷小鼠的建立和传播人类视网膜母细胞瘤肿瘤

Culturing retinoblastoma tumor cells in defined stem cell media gives rise to primary tumorspheres that can be grown and maintained for only a limited ...

科研

医学

Production and Detection of Reactive Oxygen Species (ROS) in Cancers

Production and Detection of Reactive Oxygen Species ROS in Cancers

在癌症的生产和检测活性氧（ROS）的

Reactive oxygen species include a number of molecules that damage DNA and RNA and oxidize proteins and lipids (lipid peroxydation). These reactive ...

Quantification of Atherosclerotic Plaque Activity and Vascular Inflammation using [18-F] Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography (FDG-PET/CT)

Quantification of Atherosclerotic Plaque Activity and Vascular Inflammation using [18-F] Fluorodeoxyglucose Positron Emission Tomography/Computed Tomography FDG-PET/CT

动脉粥样硬化斑块的活动和血管炎症的定量使用[18 F]，氟正电子发射断层扫描/电脑断层扫描（FDG-PET/CT）

Conventional non-invasive imaging modalities of atherosclerosis such as coronary artery calcium (CAC)1 and carotid intimal medial thickness (C-IMT)2 ...

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound

利用磁共振成像，容积电脑断层扫描及超音波的裸鼠乳腺癌骨转移大鼠模型血管生成的多模态成像

Angiogenesis is an essential feature of cancer growth and metastasis formation. In bone metastasis, angiogenic factors are pivotal for tumor cell ...

A Novel Surgical Approach for Intratracheal Administration of Bioactive Agents in a Fetal Mouse Model

一种新的手术方法气管内注射生物活性剂在胎鼠模型

Prenatal pulmonary delivery of cells, genes or pharmacologic agents could provide the basis for new therapeutic strategies for a variety of genetic and ...

Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

胆管结扎小鼠：感应炎性肝损伤和纤维化的阻塞性胆汁淤积

In most vertebrates, the liver produces bile that is necessary to emulsify absorbed fats and enable the digestion of lipids in the small intestine as well ...

In Vivo and Ex Vivo Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose

In Vivo and Ex Vivo Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose

在体内和体外途径研究银河系结构现货卵巢癌转移定植腹膜脂肪

High-grade serous ovarian cancer (HGSC), the cause of widespread peritoneal metastases, continues to have an extremely poor prognosis; fewer than 30% of ...

Quantitation of Intra-peritoneal Ovarian Cancer Metastasis

腹膜内卵巢癌转移的定量

Epithelial ovarian cancer (EOC) is the leading cause of death from gynecologic malignancy in the United States.  Mortality is due to diagnosis of 75% of ...

Development of an Alpha-synuclein Based Rat Model for Parkinson's Disease via Stereotactic Injection of a Recombinant Adeno-associated Viral Vector

通过重组腺相关病毒载体注射立体的α-突触核蛋白基于大鼠模型为帕金森氏病的发展

In order to study the molecular pathways of Parkinson&#39;s disease (PD) and to develop novel therapeutic strategies, scientific investigators rely on ...

An Orthotopic Mouse Model of Spontaneous Breast Cancer Metastasis

自发性乳腺癌转移的原位小鼠模型

Metastasis is the primary cause of mortality of breast cancer patients. The mechanism underlying cancer cell metastasis, including breast cancer ...