Name: gene regulation and targeted therapy in gastric cancer peritoneal metastasis: radiological findings from dual energy ct and pet/ct
Uploaded: 2018-01-22T14:00:00
Description: Watch this Scientific Journal Video about Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT at JoVE.com

Dette arbejde blev støttet af NSFC (nr. U1532107) og Shanghai Jiao Tong University Biomedicinsk teknik projekt (nr. YG2014MS53). Forfatterne vil gerne anerkende Jianying Li og Yan Shen for deres nyttige bemærkninger og tekniske støtte indsats i udviklingen af DECT og PET/CT billeddannelse metode.

Dette arbejde blev udført i nøje overensstemmelse med de standarder fastsat af retningslinjerne for pleje og brug af laboratoriet dyr af Shanghai Jiao Tong University og blev godkendt af laboratory Animal etiske udvalg af Ruijin Hospital.
1. mavekræft Peritoneal metastase dyremodel
<ol><li>Opdele en moderat differentieret SGC-7901 menneskelige gastrisk kræft cellelinje i en SGC-7901/sFRP1 og en SGC-7901/vektor gruppen. Kultur to grupper af celler separat i RPMI 1640 suppleret med 10% fø...

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Dyremodeller har været udbredt i studiet af molekylære mekanismer bag gastrisk kræft, og at eksperimentere med forskellige terapeutiske strategier23,24,25. I denne undersøgelse, vi har beskrevet en detaljeret protokol for mavekræft peritoneal metastase nøgen mus modellering, ved hjælp af DECT og PET/CT til billede gastrisk tumorer for at identificere tumor celledelingen i realtid og overvåge peritoneal metastaser og svar...

Gastrisk kræft (GC) forbliver den fjerde mest almindelige malignitet og den anden førende årsag til kræft dødelighed på verdensplan1. Selv om nøjagtigheden i diagnosticering og behandling af gastrisk kræft er blevet væsentligt forbedret, peritoneal metastaser er det mest centrale punkt i mavekræft prognose eller gentagelse og er en endelig determinant for postoperative død2. Det er generelt accepteret, at peritoneal formidling er en livstruende tilstand af metastaser, hvori sygdommen bliver ukontrollabel og prognosen for patienten er dårlig, når peritoneal udbredelse grundlægges. Derfor, registrering og terapeutiske effekt evaluering af gastrisk kræft peritoneal metastaser er afgørende for klinisk praksis.
Den stigende forekomst og dødelighed af gastrisk kræft havde ansporet forskere til at identificere de molekylære mekanismer. Høj udtrykket af gener som udskilles frizzled-relaterede protein 1 (sFRP1) kan føre til aktivering af signal pathway i de tidlige stadier af mavekræft, at fremme processen med tumor vækst, spredning, differentiering og apoptose3 , 4 , 5 , 6 , 7. sFRP1-overekspression celler viste en stigning i udtrykket TGFβ, downstream mål, og TGFβ-medieret EMT8. Tidligere undersøgelser har vist, at niveauet TGF-B1 er korreleret med peritoneal metastaser og TNM stadier af mavekræft. Vi har beskrevet ændringerne i kræft celledelingen reguleres af sFRP1 overekspression og TGF-B1 hæmning, og etablerede dyre modeller for peritoneal metastase viser udførelsen af tumor imaging under virkningerne af genregulering.
Dyremodeller for gastrisk kræft er uundværlige redskaber til forske tumor udvikling og eksperimentere med forskellige terapeutiske strategier uden at ofre dyr. Dyremodeller har vist sig nyttige i at studere dannelse mekanismer af tumorer og celler, fastlæggelse af tilstedeværelsen af kræftstamceller og undersøge forskellige roman terapeutiske strategier. Derfor, en real-time non-invasiv teknik kan give en nøjagtig beskrivelse af udviklingen af gastrisk tumorer og tumor respons på behandlinger, som kan identificere udviklingen af peritoneal metastase knuder i nøgen mus og overvåge ændringer af en tumor som svar på forskellige eksperimentelle og terapeutiske indgreb.
I øjeblikket, multi detektor CT (MDCT) spiller en vigtig rolle i TNM-staging af gastrisk kræft og er nyttig til at forudsige tumor resectability præoperativt9. Radiologiske undersøgelser af patienter med histologisk dokumenteret gastrisk karcinom har imidlertid primært været baseret på morfologi. DECT imaging udvider parametre for at afspejle funktionelle oplysninger ved at give monokromatiske billeder og kan være nyttige for at forbedre N iscenesættelse nøjagtighed for gastrisk kræft. Desuden, denne teknik vil aktivere erhvervelse af materiale-nedbrydning billeder, som kan være nyttigt at skelne mellem opdelte og unuanceret gastrisk karcinom, og metastatisk og ikke-metastatiske lymfeknuder10 . Med indførelsen af DECT, er den funktionelle imaging aspekt af CT også blevet tilføjet til kliniske applikationer, bidrager til evalueringer af terapeutiske virkning og forudsige patienternes prognoser11,12,13. PET/CT er en nyttig imaging teknik for påvisning og iscenesættelse af gastrisk kræft og kan vurdere recidiv af tumor effektivt14. Tumor celleproliferation og angiogenese var begge anses for at være nødvendigt i udviklingen af en påviselig tumor15, tumor knuder viste en positiv præstation med højere SUVmax på PET/CT. baseret på deres præference for aerob glykolyse, 18F-FDG, en glucose analog, er blevet udnyttet som en lovende tracer i diagnose af maligne sygdomme, kombineret med PET/CT16. Denne metode bygger på den hurtige glucose indtagelse af tumor væv og har brede kliniske anvendelser, herunder bistå i påvisning, iscenesættelse og evaluering af prognosen for tumorer, samt overvågning tumorer svar til terapi17 , 18. som ikke-invasive metoder, DECT og PET/CT har været udnyttet til at diagnosticere Maligne tumorer og vurdere tumor svar på forskellige behandlinger.
Vores gruppe har brugt denne ikke-invasive billeddannelse metode med DECT og PET/CT-scannere til at opdage og overvåge processen med tumorvækst og metastase i levende mus19. Vi udforskede billeddiagnostiske undersøgelsesresultater induceret af sFRP1-overekspression i gastrisk kræft celler i vivo bruger nøgen mus, med DECT og PET/CT, og beskrevet ændringer af den SUVmax værdi efter målrettet terapien af TGF-B1-hæmmer til at bekræfte udvikling af tumor knuder i bughinden efter gen induktion, og også studerede ændringer i tumor knuder i svar på eksperimentelle behandlinger. I dette papir præsenterer vi detaljerede procedurer for modellering gastrisk tumor peritoneal metastase i mus, og dens afsløring og overvågning med DECT og PET/CT

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			<td height="21" style="height:21px;width:247px;">Iohexol</td>
			<td style="width:389px;">BEJING BEILU PHARMACEUTICAL CO,LTD</td>
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			<td height="21" style="height:21px;width:247px;">normal saline</td>
			<td style="width:389px;">HUNAN KELUN PHARMACEUTICAL CO,LTD</td>
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			<td height="21" style="height:21px;width:247px;">BALB/c nude mice&#160;</td>
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			<td height="21" style="height:21px;width:247px;">AW Volumeshare5</td>
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			<td>dual-energy spectral CT workstation</td>
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			<td height="21" style="height:21px;width:247px;">Siemens Inveon micro-PET/CT</td>
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			<td height="21" style="height:21px;width:247px;">Inveon Acquisition Workplace</td>
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gene regulation and targeted therapy in gastric cancer peritoneal metastasis: radiological findings from dual energy ct and pet/ct

Mavekræft er stadig fjerde i kræft incidens på verdensplan med en fem-års overlevelse på kun 20% - 30%. Peritoneal metastaser er den hyppigste form for metastaser, der ledsager inoperabel gastrisk kræft og er en endelig determinant for prognosen. At forebygge og kontrollere udviklingen af peritoneal metastaser kan spille en rolle i at hjælpe til at forlænge overlevelsen af gastrisk kræftpatienter. En non-invasiv og effektiv imaging teknik vil hjælpe os til at identificere invasion og metastase processen med peritoneal metastaser og til at overvåge ændringer i tumor knuder i svar på behandlinger. Dette vil gøre det muligt for os at opnå en nøjagtig beskrivelse af udviklingsprocessen og molekylære mekanismer af gastrisk kræft. Vi har for nylig beskrevet eksperiment ved hjælp af dual energy CT (DECT) og positronemissionstomografi/beregnet tomografi (PET/CT) platforme til påvisning og overvågning af gastrisk tumor metastaser i nøgen mus modeller. Vi har vist, at ugentlige kontinuerlig overvågning med DECT og PET/CT kan identificere dynamiske ændringer i peritoneal metastaser. SFRP1-overekspression i mavekræft mus modeller viste positiv radiologiske ydeevne, en højere FDG udbredelse og stigende ekstraudstyr og SUVmax (standardiserede optagelse værdi) af knuder viste en indlysende ændring tendens i svar til målrettet behandling af TGF-B1 hæmmer. I denne artikel, vi beskrev de detaljerede non-invasiv billeddiagnostiske procedurer for at udføre mere komplekse forskning på mavekræft peritoneal metastaser ved hjælp af dyremodeller og givet repræsentative imaging resultater. Brug af ikke-invasive billeddannelse teknikker bør give os til bedre at forstå mekanismerne af tumordannelse, overvåge tumorvækst og evaluere effekten af terapeutiske interventioner for mavekræft.

DECT og PET/CT-scanning blev udført på nøgen mus efter to uger af celle linje injektioner. GSI billeder givet fremragende resultater til at vise subkutane metastaser ud over konturen af maven for gruppen sFRP1 overekspression, og metastaser med perifere enhancement blev bekræftet af farveskalaen billede (fig. 1a-c). PET/CT billeder afbildet focally unormal FDG optagelsen af metastaser, herunder i peritoneal og subkutane metastaser (<stron...

Watch this Scientific Journal Video about Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT at JoVE.com

Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT

Denne protokol beskriver værdien af dual energy CT og PET/CT billeddannelse metoder i tumor imaging og effekten evaluering. Denne artikel viser forskningsmetoder og resultater erhvervet af dual energy CT og PET/CT at evaluere genregulering og målrettet behandling af gastrisk kræft peritoneal metastaser.

RIBOREGULATION og målrettet terapi i mavekræft Peritoneal metastase: radiologiske fund fra Dual Energy CT og PET/CT

Gastric cancer remains fourth in cancer incidence worldwide with a five-year survival of only 20%-30%. Peritoneal metastasis is the most frequent type of ...

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In Vivo and Ex Vivo Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose

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