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DOI :
10.3791/56551-v
•
12:35 min
November 14th, 2017
Chapters
0:05
Title
0:55
HincII Treatment and Alkaline Hydrolysis
4:11
5'-End Phosphorylation
5:21
Single Strand DNA Ligation
6:35
Second Strand Synthesis
8:23
PCR Amplification, Library Analysis and Pooling
10:10
Results: Simultaneous Quantitation and Genome-wide Mapping of Ribonucleotides
11:39
Conclusion
在这里, 我们描述一个方法可以同时定量和基因组范围内的地图核苷酸在高度完整的 dna 在核苷酸决议, 结合酶裂解的基因组 dna 与其碱性水解和随后5´端序.
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