Visuel genkendelse af flere nukleinsyrer i en kapillær Array
November 15th, 2017
•Jianwei Chen1,2,3*, Ning Shao1,2,3*, Jiaying Hu4*, Rong Li4, Yuanshou Zhu1,2,3, Dabing Zhang4,5, Shujuan Guo1,2,3, Junhou Hui6, Peng Liu6, Litao Yang4, Sheng-Ce Tao1,2,3
1Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, 2State Key Laboratory of Oncogenes and Related Genes, 3School of Biomedical Engineering, Shanghai Jiao Tong University, 4Collaborative Innovation Center for Biosafety of GMOs, National Center for the Molecular Characterization of Genetically Modified Organisms, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 5Key Laboratory of Crop Marker-Assisted Breeding of Huaian Municipality, Jiangsu Collaborative Innovation Center of Regional Modern Agriculture and Environmental Protection, 6Department of Biomedical Engineering, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, School of Medicine, Tsinghua University
Denne protokol beskriver fabrikation af en lille, klar-til-brug kassette, der kan anvendes til visuel genkendelse af flere nukleinsyrer i en enkelt, test, der er nem at betjene. I denne tilgang, blev en kapillær array brugt til multiplex og yderst effektiv påvisning af GMO mål.
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