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DOI :
10.3791/56600-v
December 21st, 2017
Chapters
0:05
Title
0:42
Sample Fixation
2:22
Sample Permeabilization
3:10
Hybridization
4:26
Washing
5:03
Sample Preparation for Imaging
6:12
Results: Representative Results of Two Different Transcripts Labeled by the smFISH Method
7:25
Conclusion
这篇手稿描述了一种方法, 标记个别信使 RNA (mRNA) 转录与荧光标记的 DNA 探针, 用于分子荧光原位杂交 (smFISH) 实验在大肠杆菌. smFISH 是一种可视化方法, 它允许在固定的单个细胞中同时检测、定位和量化单个 mRNA 分子。
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