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DOI :
10.3791/56613-v
February 2nd, 2018
Chapters
0:05
Title
0:52
Recombinatorial Cloning to Generate Ready-for-injection Clones
2:23
Hydrodynamic Tail Vein Injection
4:22
Induction of Transfected CreER with Tamoxifen
5:33
Induction of Tetracycline-dependent Gene or shRNA Expression
6:35
Preparation of Mouse Liver for Analysis by Immunostaining
7:30
Results: Constitutive and Inducible Gene Expression in the Liver Following HTVI
8:47
Conclusion
トランスポゾン ベースの統合のベクトルの流体尾静脈注射によりマウス肝細胞の安定したトランスフェクション体内。長期的な単一遺伝子の発現や遺伝子や肝臓でミール shRNA の組み合わせ構成とドキシサイクリン誘導式ことができますトランスフェクション システムの実用的なプロトコルを紹介します。
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