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DOI :
10.3791/56615-v
December 8th, 2017
Chapters
0:05
Title
0:43
Coverslip and Mold Preparation
2:27
Preparation of Cells for Encapsulation
4:38
Cell Encapsulation
6:58
Results: Confocal Microscopy and 2D and 3D Mixed Cultures
8:47
Conclusion
在此, 我们为大鼠脑源性胶质细胞的3D 培养提供了一项协议, 包括星状细胞、小胶质和突。我们展示了原细胞培养, methacrylated 透明质酸 (哈马) 水凝胶合成, HAMAphoto 聚合和细胞封装, 和样品处理共聚焦和扫描电子显微成像。
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