Name: synthesis of monocyte-targeting peptide amphiphile micelles for imaging of atherosclerosis
Uploaded: 2017-11-17T14:00:00
Description: Watch this Scientific Journal Video about Synthesis of Monocyte-targeting Peptide Amphiphile Micelles for Imaging of Atherosclerosis at JoVE.com

作者要感谢南加州大学, 国家心脏, 肺和血液研究所 (aha), R00HL124279, Eli 和伊蒂丝广泛创新奖的财政支持, 以及力惠蒂尔基金会非癌症翻译研究奖授予 EJC。作者感谢中心的电子显微镜和微量分析, 卓越中心的 NanoBiophysics, 卓越中心的分子特性, 和翻译成像中心在南加州大学的援助仪器设置。

注: 阅读试剂的 MSDS, 并按照当地机构的要求, 遵守所有化学安全措施。
1. MCP-1 PAMs 的制备
<ol><li>
MCP-1 肽的制备
	<ol>
<li>在反应容器 (RV) 中, 称 n-芴甲氧羰基-l-赖氨酸 (Boc)-摩尔0.25。用化学油烟机冲洗5毫升 dimethylforamide (DMF) 的 RV 侧面。</li>
		<li>将 RV 加载到自动台式多肽合成器上。载入预包装的氨基酸瓶, n "-CYNFTINRKISVQRLASYRRITSS-c", 从 c 到 N 末端。在最后的脱步骤中包...

MCP-1 PAMs 是一个充满希望的分子成像平台, 包括一个亲水性靶向肽和疏水尾巴, 驱动自组装性质的纳米颗粒。这种单核细胞靶向胶束可以通过简单的合成和纯化步骤 MCP-1 肽和 DSPE-PEG (2000)-MCP-1。PAMs 有许多有益的特征为在体内分子成像, 如他们的自组装在温和的条件下, 内在生物降解性, 和结构和化学多样性允许纳入其他成像基或靶向多肽选择性地传递到特定的感兴趣的地点。它们的粒度、形状...

心血管疾病仍然是全球死亡的主要原因, 全世界约有1730万人死亡,1。心血管疾病是由动脉粥样硬化造成的, 一种情况是斑块在动脉中形成, 从而抑制血液和氧气流向身体的细胞2,3。动脉粥样硬化的进展是由炎症反应、不规则脂代谢和斑块堆积引起的动脉增厚和硬化, 导致斑块破裂和心肌梗死4,5。内皮细胞表达细胞因子和黏附分子, 其中包括 MCP-1, 结合到 c-c 趋化因子受体 (CCR2) 的表面发现单核的6,7,8。氧化胆固醇在斑块形成的早期将单核细胞转化为巨噬生物, 从而放大了该地区的炎症反应, 导致组织损伤, 形成不稳定或易受伤害的斑块9,10。
传统上, 动脉粥样硬化是评估腔狭窄的解剖成像使用血管造影或超声11,12。然而, 这些方法只能确定严重的动脉壁狭窄, 而不是动脉粥样硬化的早期阶段, 因为最初的斑块生长导致动脉重塑, 以维持动脉大小和血流率12,13, 14。因此, 造影 underrepresent 动脉粥样硬化的患病率。此外, 非侵入性成像技术, 如单光子发射计算机断层扫描, 正电子发射断层扫描和磁共振成像最近被用来表征斑块形态, 因为它们可以提供初步的细节和斑块的特征。然而, 这些模式往往受限于缺乏灵敏度, 空间分辨率, 或要求使用电离辐射, 使成像斑块进展在不同阶段更具挑战性的15,16, 17。一个成像传递系统, 将专门确定斑块在不同阶段的动脉粥样硬化仍有待开发。
纳米粒子已显示为体内斑块靶和诊断的一个新兴平台18,19,20,21。特别是, PAMs 是有利的, 因为他们的化学多样性和能力, 以适应各种基, 组成, 大小, 形状和表面功能化22。多肽分子 (PAs) 由亲水性, 多肽 "headgroup" 附着在疏水的尾巴上, 通常是脂质;这两亲结构赋予自功能, 并允许在粒子的表面上的多肽的多显示22,23,24。肽 headgroups 可通过折叠和多肽25之间的氢键作用影响颗粒形状。通过β-片相互作用折叠的多肽已经被证明形成拉长的胶束, 而α-螺旋确认可以形成球形和拉长的胶束22,23,24, 25,26,27。聚乙二醇 (PEG) 连接, 屏蔽了肽的表面电荷可以放在亲水性肽和 PAMs 的疏水尾之间, 增强了纳米颗粒在系统循环中的可用性28,29,30,31. PAMs 也很有利, 因为它们具有生物相容性, 并被证明具有广泛的应用范围3233。胶束的水溶性比其他纳米系统的优势, 如某些高分子纳米颗粒, 不溶于水, 必须在增悬浮在注射剂34。此外, 创建 PAMs 的能力, 以响应特定的刺激, 使 PAMs 一个有吸引力的候选人, 控制细胞内药物传递35。
通过结合 CCR2 受体和积累在主动脉弓, PAMs 以前开发的单核细胞靶向监测不同阶段的动脉粥样硬化病变的主动脉9。在载脂蛋白-/-小鼠中, 单核细胞积累成正比地增加到斑块级数36。此外, 发现有易破裂的晚期斑块的患者有较高的单核细胞数量37。因此, 对 PAMs 的修改, 以纳入 MCP-1 是有用的, 因为它允许更大的目标特异性和分化的早期和晚期动脉粥样硬化病变。这些概念验证研究还证实了 PAMs 是足够安全的, 可以使用 pre-clinically 并清除 renally38。由于单核细胞和炎症的特点, 其他疾病, MCP-1 PAMs 有潜力用于治疗和诊断的应用以外的其他疾病的动脉粥样硬化8,39,40,41。
在此, 我们报告了高可伸缩性和自组装 MCP-1 PAMs 的制作, 证明了粒子的最佳大小, 表面电荷, 选择性靶向单核细胞的增强成像应用在动脉粥样硬化。

<table border="0" cellpadding="0" cellspacing="0" width="834">
	<tbody>
		<tr height="20">
			<td height="20" style="height:20px;width:309px;">1,2-ethanedithiol</td>
			<td style="width:147px;">VWR</td>
			<td style="width:119px;">E0032</td>
			<td style="width:259px;">for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">10 mL disposable serological pipets</td>
			<td>VWR</td>
			<td>89130-898</td>
			<td>for cell culture</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">15 mL centrifuge tubes, polypropylene</td>
			<td>VWR</td>
			<td>89401-566</td>
			<td>for various applications</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">2,5-dihydroxybenzoic acid, 99%</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td>AC165200050</td>
			<td>for MALDI</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">25 mL disposable serological pipets</td>
			<td>VWR</td>
			<td>89130-900</td>
			<td>for cell culture</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:309px;">2-Mercaptoethanol, 50 mM</td>
			<td style="width:147px;">ThermoFisher Scientific</td>
			<td style="width:119px;">31350010</td>
			<td>for cell culture</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">5 mL disposable serological pipets</td>
			<td>VWR</td>
			<td>89130-896</td>
			<td>for cell culture</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">50 mL centrifuge tubes</td>
			<td>VWR</td>
			<td>89039-658</td>
			<td>for various applications</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:309px;">75 cm2 culture flask</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td style="width:119px;">13-680-65</td>
			<td>for cell culture</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">75 mL reaction vessel</td>
			<td>Protein Technologies</td>
			<td>3000005</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">96-wells cell culture plate</td>
			<td style="width:147px;">VWR</td>
			<td>40101-346</td>
			<td>for MTS assay</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Acetonitrile, HPLC grade</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td>A998SK-4</td>
			<td>for HPLC purification</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Borosilicate glass, 1 dram</td>
			<td style="width:147px;">VWR</td>
			<td>66011-041</td>
			<td>for PAM synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Borosillicate glass pipet, Long tips</td>
			<td style="width:147px;">VWR</td>
			<td>14673-043</td>
			<td>for various applications</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Coverslip, 0.16-0.19 mm, 22 x 22 mm</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td>12-542B</td>
			<td>for confocal microscopy</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:309px;">Cy5 amine</td>
			<td style="width:147px;">Abcam</td>
			<td style="width:119px;">ab146463</td>
			<td>for peptide conjugation</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:309px;">Diethyl ether, ACS grade</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td style="width:119px;">E138-1</td>
			<td>for peptide precipitation</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Disposable syringes, 20 mL</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td>14-817-54</td>
			<td>for HPLC purification</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Double neubauer ruled hemocytometer</td>
			<td style="width:147px;">VWR</td>
			<td>63510-13</td>
			<td>for cell counting</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:309px;">DSPE-PEG(2000) amine</td>
			<td>Avanti</td>
			<td style="width:119px;">880128P</td>
			<td>for peptide conjugation</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:309px;">DSPE-PEG(2000) maleimide</td>
			<td>Avanti</td>
			<td style="width:119px;">880126P</td>
			<td>for peptide conjugation</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">DSPE-PEG(2000)-NHS ester&#160;</td>
			<td>Nanocs</td>
			<td>PG2-DSNS-10K</td>
			<td>for conjugation to Cy5</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;width:309px;">Dulbecco&#39;s modified eagle medium-high glucose</td>
			<td style="width:147px;">Sigma Aldrich</td>
			<td style="width:119px;">D5796-500ML</td>
			<td>for cell culture</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fetal bovine serum, qualified, heat inactivated</td>
			<td>ThermoFisher Scientific</td>
			<td>10438026</td>
			<td>for cell culture</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fmoc-L-Ala-OH /HBTU</td>
			<td>Protein Technologies</td>
			<td>PS3-H5-A</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fmoc-L-Arg(Pbf)-OH /HBTU</td>
			<td>Protein Technologies</td>
			<td style="width:119px;">PS3-H5-RBF</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fmoc-L-Asn(Trt)-OH /HBTU</td>
			<td>Protein Technologies</td>
			<td style="width:119px;">PS3-H5-NT</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fmoc-L-Cys(Trt)-OH /HBTU</td>
			<td>Protein Technologies</td>
			<td style="width:119px;">PS3-H5-CT</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fmoc-L-Gln(Trt)-OH /HBTU</td>
			<td>Protein Technologies</td>
			<td>PS3-H5-QT</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fmoc-L-Ile-OH /HBTU</td>
			<td>Protein Technologies</td>
			<td style="width:119px;">PS3-H5-I</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fmoc-L-Leu-OH /HBTU</td>
			<td>Protein Technologies</td>
			<td>PS3-H5-L</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fmoc-L-Lys(Boc)-OH /HBTU</td>
			<td>Protein Technologies</td>
			<td>PS3-H5-KBC</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fmoc-L-Phe-OH /HBTU</td>
			<td>Protein Technologies</td>
			<td style="width:119px;">PS3-H5-F</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fmoc-L-Ser(tBu)-OH /HBTU</td>
			<td>Protein Technologies</td>
			<td style="width:119px;">PS3-H5-SB</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fmoc-L-Thr(tBu)-OH /HBTU</td>
			<td>Protein Technologies</td>
			<td>PS3-H5-TB</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fmoc-L-Tyr(tBu)-OH /HBTU</td>
			<td>Protein Technologies</td>
			<td>PS3-H5-YB</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Fmoc-L-Val-OH /HBTU</td>
			<td>Protein Technologies</td>
			<td>PS3-H5-V</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:309px;">Fmoc-Lys(Boc)-wang resin, 100-200 mesh</td>
			<td>Novabiochem</td>
			<td style="width:119px;">856013</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Formic acid, optima LC/MS grade</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td>A117-50</td>
			<td>for HPLC purification</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:309px;">Glycerol</td>
			<td style="width:147px;">VWR</td>
			<td style="width:119px;">M152-1L</td>
			<td>for confocal microscopy</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Hand tally counter</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td>S90189</td>
			<td>for cell counting</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Magnetic stir bars, egg-shaped</td>
			<td style="width:147px;">VWR</td>
			<td>58949-006</td>
			<td>for peptide conjugation</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Methanol, ACS certified</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td>A412-4</td>
			<td>for PAM synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">MTS cell proliferation colorimetric assay kit</td>
			<td style="width:147px;">VWR</td>
			<td style="width:119px;">10191-104</td>
			<td>for MTS assay</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">N,N-Dimethylformamide, sequencing grade</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td>BP1160-4</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">N-Methylmorpholine</td>
			<td>Protein Technologies</td>
			<td>S-1L-NMM</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:309px;">Paraformaldehyde</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td style="width:119px;">AC416780250</td>
			<td>for fixing cells</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">PBS, pH 7.4</td>
			<td style="width:147px;">ThermoFisher Scientific</td>
			<td>10010049</td>
			<td>for various applications</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Penicillin/streptomycin, 10,000 U/mL</td>
			<td>ThermoFisher Scientific</td>
			<td>15140122</td>
			<td>for cell culture</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:309px;">Peptide synthesis vessel, 25 mL&#160;</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td style="width:119px;">CG186011</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:309px;">Phosphotungstic acid&#160;</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td style="width:119px;">A248-25</td>
			<td>for TEM</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Piperidine</td>
			<td>Spectrum</td>
			<td>P1146-2.5LTGL</td>
			<td>for peptide synthesis</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Plain glass microscope slide 75 x 25 mm</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td>12-550-A3</td>
			<td>for confocal microscopy</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Reagent reservoirs, sterile&#160;</td>
			<td>VWR</td>
			<td>95128093</td>
			<td>for cell culture</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:309px;">Self-closing tweezer</td>
			<td style="width:147px;">TedPella</td>
			<td style="width:119px;">515</td>
			<td>for TEM</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">TEM support film</td>
			<td style="width:147px;">TedPella</td>
			<td style="width:119px;">01814F</td>
			<td>for TEM</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Trifluoroacetic acid&#160;</td>
			<td style="width:147px;">Fisher Scientific</td>
			<td>BP618-500</td>
			<td>for peptide cleavage and HPLC purification</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Triisopropylsilane</td>
			<td style="width:147px;">VWR</td>
			<td>TCT1533-5ml</td>
			<td>for peptide cleavage</td>
		</tr>
		<tr height="40">
			<td height="40" style="height:40px;">Trypan blue solution, 0.4%</td>
			<td style="width:147px;">ThermoFisher Scientific</td>
			<td style="width:119px;">15250061</td>
			<td>for cell counting</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">Tweezer, general purpose-serrated</td>
			<td style="width:147px;">VWR</td>
			<td>231-SA-SE</td>
			<td>for confocal microscopy</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">WEHI-274.1</td>
			<td>ATCC</td>
			<td style="width:119px;">ATCC&#160;CRL-1679</td>
			<td>murine monocyte</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;width:309px;">automated benchtop peptide synthesizer</td>
			<td>Protein Technologies</td>
			<td style="width:119px;"></td>
			<td style="width:259px;">PS3 Benchtop Peptide Synthesizer</td>
		</tr>
		<tr height="20">
			<td height="20" style="height:20px;">&#945;- cyano- 4- hydroxycinnamic acid, 99%</td>
			<td style="width:147px;">Sigma Aldrich</td>
			<td>476870-2G</td>
			<td>for MALDI</td>
		</tr>
	</tbody>
</table>

synthesis of monocyte-targeting peptide amphiphile micelles for imaging of atherosclerosis

动脉粥样硬化是心血管疾病的主要贡献者, 是全世界死亡的首要原因, 每年有1730万人丧生。动脉粥样硬化也是导致猝死和心肌梗塞的主要原因, 由不稳定的斑块引起, 破裂并咬合血管, 无预警。目前的成像模式不能区分稳定和不稳定的斑块破裂。多肽分子胶束 (PAMs) 可以克服这一缺点, 因为它们可以修改的各种靶向基, 具体绑定到病变的组织。单核细胞已被证明是动脉粥样硬化的早期标记物, 而单核细胞的大量积累与易破裂的斑块有关。因此, 能够靶向单核细胞的纳米粒子可以被用来鉴别动脉粥样硬化的不同阶段。为此, 在这里, 我们描述了一个协议为单核细胞靶向 PAMs (核细胞趋化蛋白 protein-1 (MCP-1) PAMs) 的制备。MCP-1 PAMs 是通过合成在温和条件下形成纳米颗粒的 15 nm 直径与近中性表面电荷。在体外中, PAMs 被发现具有生物相容性, 对单核细胞具有较高的亲和力. 本文所描述的方法对动脉粥样硬化以及其他炎症性疾病的广泛应用有广阔的前景。

MCP-1 PAM 的制备 MCP-1 蛋白 [YNFTNRKISVQRLASYRRITSSK] 或炒多肽 [YNSLVFRIRNSTQRKYRASIST] 的 CCR2-binding 母题 (残留 13-35) 通过在 N 端部添加半胱氨酸残留物而改变。采用全自动肽合成器, 用甲氧羰基介导的固相法合成了 MCP-1 肽。用反相高效液相色谱法在50° c 的 C8 柱上用 0.1% TFA 在乙腈/水混合物中纯化了粗肽, 并以 MALDI 质谱 (图 1) 为特征。含半胱氨酸的...

Watch this Scientific Journal Video about Synthesis of Monocyte-targeting Peptide Amphiphile Micelles for Imaging of Atherosclerosis at JoVE.com

Synthesis of Monocyte-targeting Peptide Amphiphile Micelles for Imaging of Atherosclerosis

本文介绍了一种涉及单核细胞靶向肽 amphiphile 胶束的合成和表征方法, 并对胶束与单核细胞结合的生物相容性和能力进行了相应的测试。

单核靶向肽 Amphiphile 胶束合成动脉粥样硬化的研究

Atherosclerosis is a major contributor to cardiovascular disease, the leading cause of death worldwide, which claims 17.3 million lives annually. ...

Research

JoVE Journal

Bioengineering

Cultivation of Human Neural Progenitor Cells in a 3-dimensional Self-assembling Peptide Hydrogel

在三维自组装多肽水凝胶的人类神经祖细胞的培养

The  influence of 3-dimensional (3D) scaffolds on growth, proliferation and finally  neuronal differentiation is of great interest in order to find new ...

科研

生物工程

Wideband Optical Detector of Ultrasound for Medical Imaging Applications

宽带超声医疗成像应用，光学检测仪

Optical sensors of ultrasound are a promising alternative to piezoelectric techniques, as has been recently demonstrated in the field of optoacoustic ...

Synthesis of Keratin-based Nanofiber for Biomedical Engineering

基于角蛋白纳米纤维生物医学工程合成

Electrospinning, due to its versatility and potential for applications in various fields, is being frequently used to fabricate nanofibers. Production of ...

Microwave-driven Synthesis of Iron Oxide Nanoparticles for Fast Detection of Atherosclerosis

动脉粥样硬化的快速检测的氧化铁纳米颗粒微波驱动综合

A fast and reproducible microwave-driven protocol has been developed for the synthesis of neridronate-functionalized nanoparticles. Starting from the ...

Wet Chemistry and Peptide Immobilization on Polytetrafluoroethylene for Improved Cell-adhesion

湿化学和肽固定在聚四氟乙烯为改善细胞粘附

Endowing materials surface with cell-adhesive properties is a common strategy in biomaterial research and tissue engineering. This is particularly ...

Preparation and Characterization of Lipophilic Doxorubicin Pro-drug Micelles

制备和表征亲脂阿霉素前药胶束

Micelles have been successfully used for the delivery of anticancer drugs. Amphiphilic polymers form core-shell structured micelles in an aqueous ...

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells

对细胞的超快磁化阳离子化Magnetoferritin的合成

Many important biomedical applications, such as cell imaging and remote manipulation, can be achieved by labeling cells with superparamagnetic iron oxide ...

Synthesis of Hydrogels with Antifouling Properties As Membranes for Water Purification

水凝胶的合成与防污性能作为膜的净水

Hydrogels have been widely utilized to enhance the surface hydrophilicity of membranes for water purification, increasing the antifouling properties and ...

Design and Synthesis of a Reconfigurable DNA Accordion Rack

可重构 DNA 手风琴架的设计与合成

DNA nanostructure-based mechanical systems or DNA nanomachines, which produce complex nanoscale motion in 2D and 3D in the nanometer to &#229;ngstr&#246;m ...

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

在肽膜前体的维西图洛合成中,用于自主囊泡生长

Compartmentalization of biochemical reactions is a central aspect of synthetic cells. For this purpose, peptide-based reaction compartments serve as an ...